Book ChapterDOI
An Electrochemical DNA Sensing System Using Modified Nanoparticle Probes for Detecting Methicillin-Resistant Staphylococcus aureus
TLDR
This sensing system, using a nanoparticle-modified probe, has the ability to detect 10 pM of genomic DNA from MRSA without amplification by the polymerase chain reaction.Abstract:
We have developed a novel, highly sensitive, biosensing system for detecting methicillin-resistant Staphylococcus aureus (MRSA). The system employs gold nanoparticles (AuNPs), magnetic nanoparticles (mNPs), and an electrochemical detection method. We have designed and synthesized ferrocene- and single-stranded DNA-conjugated nanoparticles that hybridize to MRSA DNA. Hybridized complexes are easily separated by taking advantage of mNPs. A current response could be obtained through the oxidation of ferrocene on the AuNP surface when a constant potential of +250 mV vs. Ag/AgCl is applied. The enzymatic reaction of L-proline dehydrogenase provides high signal amplification. This sensing system, using a nanoparticle-modified probe, has the ability to detect 10 pM of genomic DNA from MRSA without amplification by the polymerase chain reaction. Current responses are linearly related to the amount of genomic DNA in the range of 10-166 pM. Selectivity is confirmed by demonstrating that this sensing system could distinguish MRSA from Staphylococcus aureus (SA) DNA.read more
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Journal ArticleDOI
Development of novel real-time PCR assays for detection and differentiation of eleven medically important Aspergillus and Candida species in clinical specimens
Claudia Schabereiter-Gurtner,Brigitte Selitsch,Manfred Rotter,Alexander M. Hirschl,Birgit Willinger +4 more
TL;DR: In this article, real-time PCR assays targeting the fungal ITS2 region were developed for the detection and differentiation of medically important Aspergillus species (Aspergilli fumigatus), and Candida species (Candida albicans, Candida dubliniensis, Candidas glabrata, Candidia krusei, and C. tropicalis) using a LightCycler instrument.
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Detection of bacterial pathogens in municipal wastewater using an oligonucleotide microarray and real-time quantitative PCR
TL;DR: The PCR amplified DNA microarray successfully detected multiple bacterial pathogens in wastewater and a real-time quantitative PCR assay was developed based on the fluorescent TaqMan probes (Applied Biosystems).
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Development of an electrochemical DNA biosensor with a high sensitivity of fM by dendritic gold nanostructure modified electrode
Feng Li,Xiaoping Han,Shufeng Liu +2 more
TL;DR: The DenAu modified electrode could realize an ultra sensitivity of 1 fM toward complementary target DNA and a very wide dynamic detection range (from 1 f mM to 1 nM) and the whole DNA biosensor fabrication process based on the DenAU modified electrode was characterized by cyclic voltammetry and electrochemical impedance spectroscopy methods.
Journal ArticleDOI
New Methods for Detection of Campylobacters in Stool Samples in Comparison to Culture
TL;DR: The striking feature of this study is the low sensitivity of culture, in the range of 60%, in contrast to immunoenzymatic and molecular tests.
Journal ArticleDOI
Impedimetric detection of single-stranded PCR products derived from methicillin resistant Staphylococcus aureus (MRSA) isolates
Damion K. Corrigan,Holger Schulze,Grace Henihan,Ilenia Ciani,Gerard Giraud,Jonathan G. Terry,Anthony J. Walton,Ronald Pethig,Peter Ghazal,Jason Crain,Colin Campbell,Andrew R. Mount,Till T. Bachmann +12 more
TL;DR: The successful detection of long DNA fragments produced by PCR following extraction from clinical isolates is reported, which paves the way for rapid mecA detection in point of care scenarios.