Journal ArticleDOI
An evaluation of film detection methods for weak β-emitters, particularly tritium
TLDR
In this article, a sensitive film detection method for the visualization of weak β-emitters on chromatograms is reported, which allows one to visualize 2-3 nanocurie 3H/cm2/day (about 1 nanocuri/day for an average thin-layer spot).About:
This article is published in Analytical Biochemistry.The article was published on 1970-03-01. It has received 252 citations till now. The article focuses on the topics: Liquid scintillation counting.read more
Citations
More filters
Journal ArticleDOI
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
TL;DR: A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets that results in quantitative transfer of ribosomal proteins from gels containing urea.
Journal ArticleDOI
A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide Gels
TL;DR: A simple method for detecting 3H in polyacrylamide gels by scintillation autography (fluorography) using X-ray film, which is ten times more sensitive than conventional autoradiography of isotopes with higher emission energies.
Journal ArticleDOI
Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
TL;DR: It is demonstrated that pre-exposure of the film to a brief flash of light greatly increases the sensitivity of fluorography, because it corrects the non-linear relationship between radioactivity of the sample and absorbance of theFilm image.
Journal ArticleDOI
Protein blotting: Principles and applications
Jonathan M. Gershoni,G E Palade +1 more
TL;DR: Twenty-eight years ago, Smithies demonstrated that a starch gel could serve as a molecular sieve through which zone electrophoresis of proteins could be carried out and this procedure is now one of the most widely used analytical and preparative tools in cellular and molecular biology.
Journal ArticleDOI
Radioiodination of proteins in single polyacrylamide gel slices. Tryptic peptide analysis of all the major members of complex multicomponent systems using microgram quantities of total protein.
TL;DR: This technique has compared the tryptic peptides of all the major protein components of Moloney and Rauscher leukemia viruses using only 50 to 100 microgram of total protein from each virus.
References
More filters
Journal ArticleDOI
Quantitative carbon-14 and tritium assay of thin-layer chromatography plates
Fred Snyder,Nelson Stephens +1 more
TL;DR: Cab-O-Sil, a gelling agent, was used to suspend the TLC adsorbent in a liquid scintillator solution, and for this reason the procedure requires no elution steps.
Journal ArticleDOI
Liquid scintillation counting of aqueous samples
C. F. Gordon,A. L. Wolfe +1 more
TL;DR: A sample preparation method for liquid scintillation counting of aqueous suspensions or dry powders is described in this paper, where the addition of 4% by weight of Cab-O-Sil (finely divided silicon dioxide) to the liquid SCINTillation gel produces a transparent and colorless gel which is stable for 1 to 2 weeks.
Journal ArticleDOI
Low-temperature fluorography induced by tritium-labelled compounds on thin- layer chromatograms
Ursula Lüthi,P. G. Waser +1 more
TL;DR: A specific contribution of the cell genome to one of the early steps during the multiplication of influenza viruses is unlikely and there is no need for such an assumption, since the inhibitory effect of agents reacting with thecell genome might be explained by a destruction of the influenza genome in the cell nucleus without influencing the NDV multiplication in the cytoplasm.
Journal ArticleDOI
Application of filter paper partition chromatography to radioactive tracer studies.
R. M. Fink,C. E. Dent,K. Fink +2 more
TL;DR: The filter paper chromatographic procedure developed by Consden, Gordon and Martin has proved to be very useful in the qualitative and semi-quantitative analysis of amino acid mixtures, and appears to have possibilities for extension to a variety of other compounds as discussed by the authors.