Journal ArticleDOI
An immunocytochemical survey of endocrine cells in the gastrointestinal tract of chicks at hatching.
B. B. Rawdon,Ann Andrew +1 more
TLDR
The distribution of gastrin-, cholecystokinin-, glucagon, secretin, vasoactive intestinal polypeptide, glucagon and substance P was studied in samples from the following regions: proventriculus, gizzard, pylorus, duodenum, upper and lower ileum, caeca and rectum.Abstract:
The distribution of gastrin-, cholecystokinin-, glucagon-, secretin-, vasoactive intestinal polypeptide-, substance P-, bombesin-, neurotensin-, motilin-, somatostatin- and avian pancreatic polypeptide-like cells, demonstrated by indirect immunocytochemistry, was studied in samples from the following regions: proventriculus, gizzard, pylorus, duodenum, upper and lower ileum, caeca and rectum. The pylorus is particularly rich in gastrin-, neurotensin- and somatostatin-like cells. No cells immunoreactive for gastric inhibitory polypeptide or insulin were detected. In a number of instances the same cells were found to stain with antisera raised to different gut peptides. This happened with antisera detecting gastrin- and neurotensin-like cells, with antisera to avian pancreatic polypeptide and glucagon and with antisera to secretin, vasoactive intestinal polypeptide, glucagon and substance P. The possibility that antigenic determinants to more than one peptide are contained in certain endocrine-like cells is considered.read more
Citations
More filters
Journal ArticleDOI
Neurotensin: Immunohistochemical Localization in Central and Peripheral Nervous System and in Endocrine Cells and its Functional Role as Neurotransmitter and Endocrine Hormone
TL;DR: Some of the effects of NT which were previously considered to be of an endocrine or paracrine nature - such as contraction of the guinea-pig ileum - are interpreted as effects ofNT of neuronal origin.
Journal ArticleDOI
Neuronal gastrin-releasing peptide in the mammalian gut and pancreas
TL;DR: The occurrence of gastrin-releasing peptide-containing nerve cell bodies in the myenteric ganglia all along the gut indicates that gastrifying peptide fibers are intramural in origin, and suggests multiple functions of gastin-re releasing peptide, including a role in the regulation of intamural neuronal activities, smooth muscle tone and in secretory and absorptive processes.
Book
Light Microscopic Histochemistry on Plastic Sections
TL;DR: Using polar methacrylates and/or nonpolar epoxy resins as embedding media, it has been possible to demonstrate proteins and aminoacid residues, carbohydrates, lipids, nucleic acids, biogenic amines, inorganic ions, and some enzymes, although the spectrum of methods found as suitable for plastic-embedded material is far narrower than that available for paraffin or frozen sections.
Journal ArticleDOI
The embryonic origin of endocrine cells of the gastrointestinal tract.
TL;DR: It is concluded that at least the majority of gut endocrine cell types at present known are derived from the endoderm and not from the neural crest, other ectoderm, or mesoderm.
References
More filters
Journal ArticleDOI
The unlabeled antibody enzyme method of immunohistochemistry preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes:
TL;DR: PAP was heterogeneous on electrophoresis, homogeneous on sedimentation, diffusion and electron microscopy and consisted of pentagons with diameters of 205 Å, with the unexpected ratio of PO to anti-PO presumed to be due to stabilization by the pentagonal shape.
Journal ArticleDOI
Fixation of ejaculated spermatozoa for electron microscopy.
TL;DR: Osmium tetroxide fixation of crude ejaculate consistently produces a variety of artefacts such as separation of the plasma membrane from the acrosome, widening of nuclear vacuoles, erosion of the acosome, and swelling of mitochondria.
Journal ArticleDOI
A simple method for removing the resin from epoxy-embedded tissue.
TL;DR: A suitable solvent for use in tissue-resin systems is developed from the controlled chemical degrada t ion into soluble components of epoxy resins cured with phthal ic anhydride.
Journal ArticleDOI
Enkephalin-like immunoreactivity in the human gastrointestinal tract
TL;DR: Enkephalin-like immunoreactivity was present in all areas of the gut with particularly high concentration in the antrum and significant amounts in the upper small intestine.
Journal ArticleDOI
Formalin fixation for electron microscopy: a re-evaluation.
TL;DR: Several common formaldehyde fixative solutions using various buffer systems were evaluated for use with both light and electron microscopy, and ultrastructural detail was judged best using formaldehyde buffered with monobasic sodium phosphate and sodium hydroxide.
Related Papers (5)
An immunocytochemical study of the distribution of pancreatic endocrine cells in chicks, with special reference to the relationship between pancreatic polypeptide and somatostatin-immunoreactive cells.
B. B. Rawdon,Ann Andrew +1 more
Distribution, ontogeny and ultrastructure of somatostatin immunoreactive cells in the pancreas and gut.
Bifunctional reagents as vapour- and liquid-phase fixatives for immunohistochemistry.
A. G. E. Pearse,Julia M. Polak +1 more