An improved cytokinin bioassay using cultured soybean hypocotyl sections.
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TLDR
A modified soybean (Glycine max) tissue culture bioassay for cytokinins should prove to have several advantages over the conventional soybean callus bioassays including convenience, lower variability between tissue samples, and improved resolution.Abstract:
This paper describes a modified soybean (Glycine max) tissue culture bioassay for cytokinins. Soybean hypocotyls were grown under sterile conditions and sliced into 1-mm sections. Sections were cultured for 5,9,13, or 22 days on a callus medium with zeatin or other cytokinins. The fresh weight of sections increased with the cytokinin concentration from 0.0005 to 1 mum zeatin; 2-fold concentration differences were readily distinguishable at 9 days. The assay should prove to have several advantages over the conventional soybean callus bioassay including convenience, lower variability between tissue samples, and improved resolution. Its specificity is comparable to that of the soybean callus bioassay.read more
Citations
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Book ChapterDOI
Functions of Hormones at the Cellular Level of Organization
TL;DR: In this chapter the emphasis will be on the hormonal regulation of cell development, while recognizing that the processes involved are strongly influenced by both the physical constraints and metabolic activities of neighboring cells in complex tissues.
Journal ArticleDOI
Cytokinin metabolism in Phaseolus vulgaris L. - Identification of endogenous cytokinins and metabolism of [8-14C]dihydrozeatin in stems of decapitated plants
TL;DR: The major cytokinins in stems of decapitated, disbudded bean plants have been identified by enzymic degradation, Sephadex LH20 and reversed phase high performance liquid chromatography, and by combined gas chromatography-mass spectrometry as zeatin ribotide-O-β-D-glucoside and dihydrozeatin ribotide.
Book ChapterDOI
Quantitative Analysis of Plant Hormones
David R. Reeve,Alan Crozier +1 more
TL;DR: The relevance of such estimates to the true hormonal status of the plant at the time of extraction is open to question and will undoubtedly be an area of much future investigation as mentioned in this paper, however, in the absence of pertinent data the scope of this article will have to be confined to questions relating to the analysis of plant extracts.
Journal ArticleDOI
The effect of drought on levels of abscisic acid, cytokinins, gibberellins and ethylene in aeroponically-grown sunflower plants
TL;DR: The levels of cytokinins in the shoots of droughted plants were about half those in unstressed shoots, and qualitative differences occurred in the roots, and the effect of drought on GAs and ethylene was minimal.
Journal ArticleDOI
The role of plant hormones and carbohydrates in the growth and survival of coppiced Eucalyptus seedlings
TL;DR: Decapitation did not markedly alter the relatively high amounts of GAs in roots and shoots of E. obliqua, the susceptible species, although increased levels of Gas were observed in the stumps of seedlings left with 1 shoot after decapitation.
References
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Book ChapterDOI
Kinetin and Kinetin-Like Compounds
TL;DR: Compounds which strongly promote cell division are detected in numerous plant extracts such as those from coconuts, maize endosperm, banana and horsechestnut fruits, carrot and tobacco leaves, grapes, crown gall tumours, female gametophytes of Ginkgo and many other sources.
Journal ArticleDOI
Regulators of cell division in plant tissues : V. A comparison of the activities of zeatin and other cytokinins in five bioassays.
TL;DR: The carrot-root tissue culture assay for cytokinin activity has been improved by changing the site of explant excision and eliminating certain vitamins from the basal medium, which increased its sensitivity and enabled zeatin to be detected at concentrations less than 5×10-5μM.
Journal ArticleDOI
Hormonal Control of Cell Proliferation and Xylem Differentiation in Cultured Tissues of Glycine max var. Biloxi
D. E. Fosket,John G. Torrey +1 more
TL;DR: Neither NAA nor kinetin at any concentration tested stimulated tracheary element formation in the absence of an effective level of the other hormone, however, 2,4-D at 10(-7) or 10(-6)m promoted both cell proliferation and tracheARY element differentiation in the presence of an exogenous cytokinin.
Journal ArticleDOI
Modification of logarithmic growth rates of tobacco callus tissue by gibberellic Acid.
TL;DR: Logarithmic growth rates of tobacco callus tissues grown on 10(-4) to 10(-1) mum cytokinin are increased if gibberellic acid (10(-3)-2 mum) is incorporated into the medium, and the maximum dry weight yield, although achieved sooner than controls, does not differ appreciably from yields of tissue not treated with gibb Berellic Acid.
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