Journal ArticleDOI
An Investigation of the Conformational Changes of Histones F1 and F2a1 by Proton Magnetic Resonance Spectroscopy
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TLDR
High resolution nuclear magnetic resonance spectroscopy is used to study conformational changes in histone fractions F2a1 and F1 when the ionic strength of aqueous solutions is raised and the proportion of amino acid residues incorporated into secondary structure is less than that involved in the conformationalChanges indicated by thenuclear magnetic resonance results.Abstract:
High resolution nuclear magnetic resonance spectroscopy is used to study conformational changes in histone fractions F2a1 and F1 when the ionic strength of aqueous solutions is raised. Increasing line widths of certain resonance peaks, in particular those of apolar and aromatic amino acids, together with sequence data lead to the conclusion that the C-terminal half of F2a1 and a central portion of F1 are involved in the conformational changes. The proportion of amino acid residues incorporated into secondary structure (as indicated by optical rotatory dispersion) is less than that involved in the conformational changes indicated by the nuclear magnetic resonance results. Intermolecular interactions are therefore postulated to explain this difference and these are specific in as much as they involve only a part of the histone molecule and include the regions of the chain having high potential for secondary structure formation.read more
Citations
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Journal ArticleDOI
Functional anthology of intrinsic disorder. 1. Biological processes and functions of proteins with long disordered regions.
Hongbo Xie,Slobodan Vucetic,Lilia M. Iakoucheva,Christopher J. Oldfield,A. Keith Dunker,Vladimir N. Uversky,Zoran Obradovic +6 more
TL;DR: This work opens a series of three papers, which enriches the current view of protein structure-function relationships, especially with regards to functionalities of intrinsically disordered proteins, and provides researchers with a novel tool that could be used to improve the understanding of the relationships between protein structure and function.
Journal ArticleDOI
Guiding protein aggregation with macromolecular crowding.
TL;DR: How macromolecular crowding guides protein between different aggregation pathways is studied by analyzing the effect of crowding agents on the aggregation patterns under the variety of conditions favoring different aggregated end products in diluted solutions.
Journal ArticleDOI
A model for particulate structure in chromatin
TL;DR: A model is proposed for the structure of nuclease-resistant chromatin particles that proposes that the DNA in such a particle is wound about a protein core, made up of the hydrophobic regions of histone molecules.
Journal ArticleDOI
Purification of the five main calf thymus histone fractions by gel exclusion chromatography
TL;DR: Hydrophobic interactions be tween pep~ide chains, and pIoty! delerm~aed conformationa] features may affect (flue behavJour :of histones in gel p,0re~ ~7] and may be ie~p0u~ihle for the unsatisfactory separation, of F3, F2a 2 and F2b.
Journal ArticleDOI
Magnetic resonance studies of deoxyribonucleoprotein.
TL;DR: Of the five major types of histone fraction normally associated with mammalian genetic material, the lysine-rich histone F1 (or histone I) has some properties which set it apart from the others, and a proposed scheme for this interaction, involving an antiparallel alignment of chain segments has been presented.
References
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Journal ArticleDOI
Use of Helical Wheels to Represent the Structures of Proteins and to Identify Segments with Helical Potential
TL;DR: The three-dimensional structures of alpha-helices can be represented by two-dimensional projections which the authors call helical wheels, and these wheels can be used to divide sequences into segments with either helical or nonhelical potential.
Journal ArticleDOI
Studies on histones. 7. Preparative methods for histone fractions from calf thymus.
Journal ArticleDOI
Structure and function of haemoglobin: II. Some relations between polypeptide chain configuration and amino acid sequence
TL;DR: In a protein of unknown structure, a regular periodicity of invariant non-polar sites might serve to recognize helical regions, and the incidence of prolines to define their lengths, if all the proline sites observed are plotted along the sequence.
Journal ArticleDOI
Calf and Pea Histone IV II. THE COMPLETE AMINO ACID SEQUENCE OF CALF THYMUS HISTONE IV; PRESENCE OF ε-N-ACETYLLYSINE
Robert J. DeLange,Robert J. DeLange,Douglas M. Fambrough,Douglas M. Fambrough,Emil L. Smith,Emil L. Smith,James Bonner,James Bonner +7 more
TL;DR: The distribution of residues of various types in histone IV is uneven and suggests a high degree of specificity and a unique conformation, which suggests that the NH2-terminal part of the histone may involve the major binding sites to DNA whereas the COOH-terminAL region appears to be capable of possessing a specific protein conformation.
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