Journal ArticleDOI
Anti-inflammatory, gastroprotective, and cytotoxic effects of Sideritis scardica extracts.
Vanja Tadić,Ivica Jeremic,Silva Dobrić,Aleksandra Isakovic,Ivanka Markovic,Vladimir Trajkovic,Dragica Bojović,Ivana Arsić +7 more
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TLDR
It seems that phenolic compounds (apigenin, luteolin, and their corresponding glycosides) are responsible for the diethyl ether extract cytotoxic effect, and induction of oxidative stress might be involved in its cytotoxicity.Abstract:
Sideritis scardica Griseb (ironwort, mountain tea), an endemic plant of the Balkan Peninsula, has been used in traditional medicine in the treatment of gastrointestinal complaints, inflammation, and rheumatic disorders This study aimed to evaluate its gastroprotective and anti-inflammatory activities Besides, continuously increasing interest in assessing the role of the plant active constituents preventing the risk of cancer was a reason to make a detailed examination of the investigated ethanol, diethyl ether, ethyl acetate, and N-butanol extracts regarding cytotoxicity Oral administration of the investigated extracts caused a dose-dependent anti-inflammatory effect in a model of carrageenan-induced rat paw edema Gastroprotective activity of the extracts was investigated using an ethanol-induced acute stress ulcer in rats The cytotoxic activity of plant extracts was assessed on PBMC, B16, and HL-60 cells and compared to the cytotoxicity of phenolic compounds identified in extracts Apoptotic and necrotic cell death were analyzed by double staining with fluoresceinisothiocyanate (FITC)-conjugated annexin V and PI The developed HPLC method enabled qualitative fingerprint analysis of phenolic compounds in the investigated extracts Compared to the effect of the positive control, the anti-inflammatory drug indomethacine (4 mg/kg), which produced a 50 % decrease in inflammation, diethyl ether and N-butanol extracts exhibited about the same effect in doses of 200 and 100 mg/kg (536 and 487 %; 484 and 499 %, respectively) All investigated extracts produced dose-dependent gastroprotective activity with the efficacy comparable to that of the reference drug ranitidine The diethyl ether extract showed significant dose-dependent cytotoxicity on B16 cells and HL-60 cells, decreasing cell growth to 513 % and 775 % of control, respectively, when used at 100 µg/mL It seems that phenolic compounds (apigenin, luteolin, and their corresponding glycosides) are responsible for the diethyl ether extract cytotoxic effect It also appears that induction of oxidative stress might be involved in its cytotoxicity, since B16 and HL-60 cells increased their ROS production in response to treatment with diethyl ether extract Neither of the tested extracts nor any phenolic compounds showed significant cytotoxic effect to human PBMC These results demonstrated the potent anti-inflammatory and gastroprotective activities, as well as the promising cytotoxicityread more
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Medicinal Plants of the Family Lamiaceae in Pain Therapy: A Review.
Cristina M. Uritu,Cosmin T. Mihai,Gabriela Dumitrita Stanciu,Gianina Dodi,Teodora Alexa-Stratulat,Andrei Luca,Maria Magdalena Leon-Constantin,Raluca Stefanescu,Veronica Bild,S. Melnic,Bogdan Ionel Tamba +10 more
TL;DR: The data highlighted in this review paper provide valuable scientific information for the specific implications of Lamiaceae plants in pain modulation that might be used for isolation of potentially active compounds from some of these medicinal plants in future and formulation of commercial therapeutic agents.
Journal ArticleDOI
Medicinal Plants of the Family Lamiaceaeas Functional Foods – a Review
Klaudija Carović-Stanko,Marko Petek,Grdiša Martina,Jasna Pintar,Dalibor Bedeković,Mirjana Herak Ćustić,Zlatko Šatović +6 more
TL;DR: This review focuses on the Lamiaceae species and their secondary metabolites encompassing a wide array of beneficial functions and their applicability as sources of functional foods and could help in addressing specific consumer needs as healthy diet is a part of the lifestyle that maintains or improves overall health.
Journal ArticleDOI
Sideritis scardica Griseb., an endemic species of Balkan peninsula: Traditional uses, cultivation, chemical composition, biological activity
TL;DR: This review aims to provide a comprehensive overview of the traditional use, phytochemistry, biological activity, cultivation, and extraction of Sideritis scardica and to highlight the gaps in knowledge which deserves further research.
Journal ArticleDOI
In vitro antibacterial and cytotoxic activity of leaf extracts of Centella asiatica (L.) Urb , Warburgia salutaris (Bertol. F.) Chiov and Curtisia dentata (Burm. F.) C.A.Sm - medicinal plants used in South Africa
TL;DR: Overall, the extracts had good antibacterial activity and anti-proliferative effects against selected cancerous cell lines and may act as an immune booster and prevent infection in immunosuppressed cancer patients.
Journal ArticleDOI
Chemical and antimicrobial evaluation of supercritical and conventional Sideritis scardica Griseb., Lamiaceae extracts.
Vanja Tadić,Dragica Bojović,Ivana Arsić,Sofija Đorđević,Ksenija Aksentijevic,Marko Stamenić,Slobodan M. Jankovic +6 more
TL;DR: A comparison between conventional and alternative extraction methods regarding the qualitative and quantitative composition of the obtained extracts as analyzed by GC and GC-MS techniques and their anitimicrobial activity is reported.
References
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Antioxidant Determinations by the Use of a Stable Free Radical
TL;DR: In this paper, the chemical nature of the antioxidant is known and a test specific for the compound or group of interest; for example, the nitroprusside test for sulphydryl groups.
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Structure-antioxidant activity relationships of flavonoids and phenolic acids
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Antioxidant Activity and Total Phenolics in Selected Fruits, Vegetables, and Grain Products
TL;DR: In this article, the antioxidant activity and total phenolics of 28 plant products, including sunflower seeds, flaxseeds, wheat germ, buckwheat, and several fruits, vegetables, and medicinal plants were determined.
Journal ArticleDOI
Consequences of cell death: exposure to necrotic tumor cells, but not primary tissue cells or apoptotic cells, induces the maturation of immunostimulatory dendritic cells.
Birthe Sauter,Matthew L. Albert,Loise M. Francisco,Marie Larsson,Selin Somersan,Nina Bhardwaj +5 more
TL;DR: In this article, the authors investigated whether exposure to apoptotic or necrotic cells affected dendritic cells' maturation and found that only exposure to the latter induces maturation.
Journal ArticleDOI
More than one way to die: apoptosis, necrosis and reactive oxygen damage
TL;DR: The distinguishing features of the various cell death pathways are reviewed: caspases (cysteine proteases cleaving after particular aspartate residues), mitochondria and/or reactive oxygen species are often, but not always, key components.