Antibacterial activity of Mangrove Medicinal Plants against Gram positive Bacterial pathogens

TLDR: Minimum inhibitory concentration (MIC) of the mangrove plant extracts against bacterial isolates was tested in Mueller Hinton broth by Broth macro dilution method and showed minimum inhibition zone against bacterial pathogens.

Abstract: Ten mangrove medicinal plants viz., Avicennia marina, Rhizophora mucuronata , Rhizophora mangle, Asparagus officinalis, Ceriops decandra, Aegiceras corniculatum, Acanthus ilicifolius, Bruguiera cylindrica, Rhizophora apiculata and Xylocarpus grantum were collected from mangrove forest of Pichavaram, Tamil Nadu, India. The antibacterial activity of mangrove plant extracts (150 mg/ml and 300 mg/ml) were determined by Disc diffusion method. The zone of inhibition was more at 300 mg/ml of extracts when compared to 150 mg/ml of extracts. The antibacterial activity of selecte d mangrove plant leaf extracts was determined against pathogenic bacterial isolates. The methanol extract of Ceriops decandrashowed maximum zone of inhibition against all the bacterial isolates followed by Avicennia marina, Rhizophora mucronata , Aegiceras corniculatum, Rhizophora apiculata, Rhizophora mangle , Acanthus ilicifolius, Asparagus officinalis, Xylocarpus grantumand Bruguiera cylindrica at 300 mg/ml. The hexane extract of mangrove plants showed minimum inhibition zone against bacterial pathogens when compared to the other solvent extracts. The DMSO was used as a blind control and the antibiotic Ampicillin (300 mg/ml) was used as a positive control. Minimum inhibitory concentration (MIC) of the mangrove plant extracts against bacterial isolates was tested in Mueller Hinton broth by Broth macro dilution method. The MIC of mangrove plants against bacterial pathogens was ranged between 20 mg/ml to 640 mg/ml.