Journal ArticleDOI
Anticancer potential against cervix cancer (HeLa) cell line of probiotic Lactobacillus casei and Lactobacillus paracasei strains isolated from human breast milk
Muhammad Shahid Riaz Rajoka,Haobin Zhao,Yao Lu,Ziyang Lian,Na Li,Nazim Hussain,Dongyan Shao,Mingliang Jin,Qi Li,Junling Shi +9 more
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TLDR
The results indicate that the Lactobacillus strains isolated from human breast milk could be considered as a topical medication with a potential therapeutic index due to their efficacy against cervix cancer cells.Abstract:
Lactic acid bacteria have been categorized as probiotics and play a crucial role in human health by stimulating the supply of nutrients, shaping the immune system, and preventing the colonization of pathogenic microbes. This study investigated the mechanisms for the action of three potential probiotic Lactobacillus strains: Lactobacillus casei SR1, Lactobacillus casei SR2, and Lactobacillus paracasei SR4 isolated from human breast milk. These Lactobacillus strains were identified via 16S DNA sequencing and characterized via biochemical assays including acid resistance, bile resistance, antioxidant activity, and antibiotic susceptibility. The bioactivity of the cell-free culture supernatant (CFCS) secreted by these strains on the cervix cancer (HeLa) cell line was also evaluated via cytotoxicity assay and apoptosis analysis. The mechanism of anticancer activity was also investigated via RT-qPCR and western blotting. The results demonstrated that these newly isolated Lactobacillus strains from human milk displayed noticeable probiotic characteristics such as excellent antibiotic susceptibility, outstanding antioxidant activity, and promising resistance to low pH and high concentration of bile salts. The results of the conducted bioactivity assays verified that the CFCSs had acceptable anticancer effects on cervix cancer (HeLa) cells by upregulating the expression of apoptotic genes BAX, BAD, caspase3, caspase8, and caspase9 and by downregulating the expression of the BCl-2 gene. Overall, these results indicate that the Lactobacillus strains isolated from human breast milk could be considered as a topical medication with a potential therapeutic index due to their efficacy against cervix cancer cells.read more
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Food-Origin Lactic Acid Bacteria May Exhibit Probiotic Properties: Review
TL;DR: In light of extensive new scientific evidence, should the possibility of changing the current FAO/WHO requirements for the definition of probiotic bacteria be considered?
Journal ArticleDOI
Postbiotic metabolites produced by Lactobacillus plantarum strains exert selective cytotoxicity effects on cancer cells
Li-Oon Chuah,Hooi Ling Foo,Teck Chwen Loh,Noorjahan Banu Alitheen,Swee Keong Yeap,Nur Elina Abdul Mutalib,Raha Abdul Rahim,Khatijah Yusoff +7 more
TL;DR: PM produced by the six strains of L. plantarum exhibited selective cytotoxic via antiproliferative effect and induction of apoptosis against malignant cancer cells in a strain-specific and cancer cell type-specific manner whilst sparing the normal cells.
Journal ArticleDOI
Characterization and anti-tumor activity of exopolysaccharide produced by Lactobacillus kefiri isolated from Chinese kefir grains
Muhammad Shahid Riaz Rajoka,Hafiza Mahreen Mehwish,Huiyan Fang,Arshad Ahmed Padhiar,Xierong Zeng,Mohsin Khurshid,Zhendan He,Liqing Zhao +7 more
TL;DR: The present results suggested that the exopolysaccharide from Lactobacillus kefiri MSR101 is not only used in the functional food product but also considered as a topical medication due to their effectiveness against colon cancer.
Journal ArticleDOI
Characterization of potentially probiotic lactic acid bacteria and bifidobacteria isolated from human colostrum.
Wenjun Liu,Meixuan Chen,Lana Duo,Jicheng Wang,Shuai Guo,Haotian Sun,Bilige Menghe,Heping Zhang +7 more
TL;DR: This study isolated and identified lactic acid bacteria (LAB) from human colostrum, and screened 2 strains with probiotic potential, which have the potential for application as probiotics and will facilitate functional studies of probiotics in breast milk and the development of human milk-derived probiotics.
Journal ArticleDOI
Probiotic potential and biofilm inhibitory activity of Lactobacillus casei group strains isolated from infant feces
Armin Tarrah,Vinícius da Silva Duarte,Juliana de Castilhos,Shadi Pakroo,Wilson José Fernandes Lemos Junior,Rosa Helena Luchese,André Fioravante Guerra,Rochele Cassanta Rossi,Denize Righetto Ziegler,Viviana Corich,Alessio Giacomini +10 more
TL;DR: Eight Lactobacillus strains, newly isolated from infant feces, were investigated for the presence of probiotic properties such as antimicrobial susceptibility, hemolytic activity, resistance to simulated gastrointestinal conditions, bile salts hydrolyticActivity, inhibitory ability against biofilm formation by other bacteria, attachment to HT-29 human cancer cells and anti-cancer activity.
References
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