Journal ArticleDOI
Application of a modified Coomassie brilliant blue protein assay in the study of protein adsorption on carbon thin films
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In this paper, a modified Coomassie brilliant blue (CBB) protein assay (or Bradford method) was used to detect the amount of proteins adsorbed on the material surfaces after the pilot research for the optimal ratio of protein-to CBB-solution had been performed.Abstract:
The aim of this study is to investigate the adsorptive property of proteins on surfaces of three kinds of carbon films (DLC, CN 0.088 , and CN 0.15 ) and the medical origin of PMMA. The carbon films were first synthesized by magnetron sputtering with different deposition parameters. The surface characteristics of both the carbon films and the original samples were then measured using a contact-angle meter. A modified Coomassie brilliant blue (CBB) protein assay (or Bradford method) was used to detect the amount of proteins adsorbed on the material surfaces after the pilot research for the optimal ratio of protein- to CBB-solution had been performed. The results of the pilot research show that the optimal ratio of protein- to CBB-solution is 1:3, and the protein adsorption results show that the sequence amounts of proteins adsorbed on four kinds of surfaces are DLC > CN 0.088 > PMMA > CN 0.15 . This was the same as the hydrophobicity sequence of four kinds of surfaces obtained from the contact-angle test. However, the ratio of albumin to IgG ( R A/I ) adsorbed on the four surfaces has an order of PMMA > CN 0.15 > DLC > CN 0.088 , which indicates the anti-thrombogenicity property of the four kinds of surfaces.read more
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References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
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Protein adsorption and inactivation on surfaces. Influence of heterogeneities
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Surface Properties of Polystyrene Nanoparticles Coated with Dextrans and Dextran−PEO Copolymers. Effect of Polymer Architecture on Protein Adsorption
TL;DR: In this paper, hydrophobically substituted dextran copolymers (DexP−PEO) have been used to modify the surface of polystyrene latex particles, which has been shown to be useful in the field of medical applications.
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Incorporating α-Allyl Glucoside into Polyacrylonitrile by Water-Phase Precipitation Copolymerization To Reduce Protein Adsorption and Cell Adhesion
TL;DR: In this article, α-Allyl glucoside (AG) was incorporated into polyacrylonitrile by water-phase precipitation copolymerization (WPPCP) for the first time with K2S2O8−Na2SO3 as initiator system to improve the resistance properties of protein adsorption and cell adhesion for acrylitrile-based polymer.