Application of Molecular Microbiology Techniques in Bioremediation of Hydrocarbons and Other Pollutants
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TLDR
The application of molecular microbiology techniques Studying microbial populations in polluted sites without the need for culturing has led to the discovery of novel and unrecog nized populations.Abstract:
Molecular microbiology techniques have revolutionized microbial ecology by paving the way for rapid, high -throughput methods for culture -independent assessment and exploitation of microbial communities present in complex ecosystems like crude oil/hydrocarbon polluted soil. The soil microbial community is relatively diversewith a high level of prokaryotic diversity. This soil species pool represents a gold mine for genes involved in the biodegradation of different classes of pollutants. Currently, less than 1% of this diversity is culturable by traditional cultivation techniques. The application of molecular microbiology techniquesinstudyingmicrobial populations in polluted sites without the need for culturing has led to the discovery of novel and unrecog nizedread more
Citations
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Microbial Population Changes During Bioremediation of an Experimental Oil Spill
Y. J. Chang,Gregory A. Davis,Sarah J. Macnaughton,John R. Stephen,Albert D. Venosa,David C. White +5 more
TL;DR: PLFA analysis indicated that by week 14 the microbial community structures of the oiled plots were becoming similar to those of the unoiled controls from the same time point, but DGGE analysis suggested that major differences in the bacterial communities remained.
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Metatranscriptome analysis deciphers multifunctional genes and enzymes linked with the degradation of aromatic compounds and pesticides in the wheat rhizosphere
TL;DR: Abundance of the transcripts related to the degradation of aromatic amin compounds, carbazoles, benzoates, naphthalene, ketoadipate pathway, phenols, biphenyls and xenobiotics indicated abundant degradation capabilities in the soils.
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Culture-dependent characterization of hydrocarbon utilizing bacteria in selected crude oil-impacted sites in Bodo, Ogoniland, Nigeria
TL;DR: In this paper, an investigation was carried out to characterize microbial communities in selected crude oil polluted sites in Bodo community, Gokana Local Government Area of Rivers State, Nigeria, which revealed that there is a high population of active indigenous hydrocarbon utilizing bacteria which can be monitored and enhanced to bring about bioremediation in the study area.
Full Length Research Paper - Enhancement of recovery of residual oil using a biosurfactant slug
G. C. Okpokwasili,A. A. Ibiene +1 more
TL;DR: Results suggest that biosurfactant produced by Pseudomonas species is a potential “candidate” for microbially enhanced oil recovery.
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Molecular characterization of autochthonous hydrocarbon utilizing bacteria in oil-polluted sites at Bodo Community, Ogoni land, Niger Delta, Nigeria
TL;DR: It is highly recommended that16S rRNA-gene-based techniques be used when studying the bacterial community diversity and dynamics in oil-polluted ecosystems in order to fully describe the active microorganisms involved in hydrocarbon degradation.
References
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