Open AccessJournal Article
Assessment of the Mechanism of the Leukocyte Adherence Inhibition Test
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TLDR
A role for serum factors in the mechanism of LAI reactivity is suggested and partially explains the participation of multiple cell types in the responses observed, including naive cells and T-lymphocytes.Abstract:
This study was designed to elucidate the mechanism of the leukocyte adherence inhibition (LAI) test in man. To identify the reactive cell types, enriched leukocyte populations (dextran-separated leukocytes and Hypaque-Ficoll-isolated mononuclear cells and neutrophils, as well as rosette-isolated B- and T-lymphocytes) were tested for leukocyte adherence in the absence of serum to tumor-specific antigens. LAI reactivity was not restricted to any of the enriched populations, suggesting the involvement of multiple cell types. Attempts to demonstrate soluble lymphocyte factors in the LAI mechanism have been uniformly negative. In contrast, factors in serum of immune donors were able to arm naive cells to be specifically responsive. This suggests a role for serum factors in the mechanism of LAI reactivity and partially explains the participation of multiple cell types in the responses observed. In additional studies, we could not document a correlation between the magnitude of the dermal test (delayed cutaneous hypersensitivity) and the magnitude of the LAI response in patients with squamous cell carcinoma of the head and neck. In 34 of 54 of these patients, there was agreement between the two tests (both positive, 27 of 54; both negative, 7 of 54). In the remaining 20 patients, the dermal test was >5 mm while the LAI test was negative (<30% inhibition).read more
Citations
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Effect of tin on immune responses of mice
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The cellular mechanisms involved in leucocyte adherence inhibition (LAI) tests of patients with breast cancer
TL;DR: The results suggest that the LAI test measured cell-mediated immunity to tumor-type-specific antigens, and Indomethacin-sensitive inhibitors might account for some previous failures to detect a soluble adherence inhibition factor (LAIF) as part of a specific immune response.
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Leukocyte adherence inhibition test: Cellular requirements for the elaboration of a polymorph adherence inhibition activity
TL;DR: It is suggested that the LAI test involves a sequential mechanism leading to the activation of PMN in such a manner that they lose their adherence to glass.
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A Novel Utility for an Old Method: The Leukocyte Adherence Inhibition Test Is an Easy Way to Detect the Immunoreactive Interference of the Collection Tube Anticoagulant on Cellular Immunoassays
Celso Eduardo Olivier,Raquel Acácia Pereira Gonçalves dos Santos,Regiane Patussi dos Santos Lima,Daiana Guedes Pinto Argentão,Grayce Katlen Moreno da Silva,Mariana Dias da Silva +5 more
TL;DR: The leukocyte adherence inhibition (LAI) test may be used as a preanalytical tool for choosing the less immunoreactive anticoagulant for more complex immunoassays.
References
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Journal Article
Cell-mediated Antitumor Immunity in Breast Cancer Patients Evaluated by Antigen-induced Leukocyte Adherence Inhibition in Test Tubes
N. Grosser,D. M. P. Thomson +1 more
TL;DR: The assay described is a comparatively simple and sensitive technique for demonstrating cell-mediated antitumor immunity and appears to be immunologically specific.
Journal ArticleDOI
Antigen-mediated macrophage adherence inhibition
TL;DR: Antigen-mediated macrophage adherence inhibition (MAI) was studied in inbred rats immunized with various transplantation, tumour-specific and protein antigens and seems to be due to the direct interaction of the respective antigen with a corresponding PC receptor.
Journal ArticleDOI
Tube leukocyte adherence inhibition assay for the detection of anti-tumour immunity. II. Monocyte reacts with tumour antigen via cytophilic anti-tumour antibody.
TL;DR: Normal peripheral blood monocytes could be made specifically reactive (“armed”) to the tumour extract by incubating normal peripheral blood leukocytes with serum from a reactive cancer patient, and IgG isolated from “arming” sera was shown to have the capacity to sensitize normal leukocyte.
Journal ArticleDOI
Tube leukocyte (monocyte) adherence inhibition assay for the detection of anti-tumour immunity. III. "Blockade" of monocyte reactivity by excess free antigen and immune complexes in advanced cancer patients.
N. Grosser,D. M. P. Thomson +1 more
TL;DR: Non‐reactivity in the tube LAI assay of patients with metastatic cancer was not the result of a numerical deficit of circulating monocytes but was mediated by an excess of tumour antigen in the microenvironment of the sensitized monocyte.
Journal Article
Functional activities of rosette separated human peripheral blood leukocytes.
Jack H. Dean,John S. Silva,J. L. McCoy,Connie M. Leonard,Grace B. Cannon,Ronald B. Herberman +5 more
TL;DR: Subpopulations of human peripheral blood leukocytes were isolated by rosette formation and tested for functional activity, and a staphylococcal antigen preparation triggered lymphoproliferative reactivity in the E- RFC, E-RFC depleted, EAC-RFC, and the null cell subpopulations.
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