Journal ArticleDOI
Assignment of proton and carbon NMR signals of didemnin A in solution
Reads0
Chats0
TLDR
Several one-and two-dimensional techniques have been used to assign the 1H and 13C NMR spectra of the cyclic heptadepsipeptide didemnin A in DMSO as discussed by the authors.Abstract:
Several one- and two-dimensional techniques have been used to assign the 1H and 13C NMR spectra of the cyclic heptadepsipeptide didemnin A in DMSO. The composition and sequence of the amino acid residues was known beforehand and could be confirmed by heteronuclear proton–carbon long-range couplings using the COLOC experiment and a recently proposed new inverse technique using Gaussian-shaped pulses. The aliphatic methyl resonances of the isostatin, hydroxyisovalerylpropionyl, N-methylleucine and leucine residues were assigned by means of the TOCSY and NOESY techniques. Aliphatic carbon assignment was performed by inverse correlation via heteronuclear double quantum coherence.read more
Citations
More filters
Journal ArticleDOI
Natural products as probes of cell biology: 20 years of didemnin research.
TL;DR: The didemnin field illustrates how natural product chemistry may be used as a critical tool for the study of cell biology, with work directed towards understanding how this group of natural products interact with fundamental processes such as cell proliferation, protein biosynthesis, and apoptosis.
Journal ArticleDOI
Conformational Analysis of Didemnins. A multidisciplinary approach by means of X-Ray, NMR, molecular-dynamics, and molecular-mechanics techniques
TL;DR: In this paper, the authors present the application of several homo-and heteronuclear 1D and 2D-NMR techniques to assign the 1HNMR chemical shifts of the dominant conformation of didemnin B (2; three different conformations in (D6)DMSO solution in the ratio 8:1:1):1) and its conformational analysis, as well as the solution conformation, and the conformations were refined by restrained molecular-dynamics calculations using the GROMOS program and by MOMO, a novel
Book ChapterDOI
Chapter 21. Macrocyclic Immunomodulators
Craig E. Caufield,John H. Musser +1 more
TL;DR: FK-506, RPM, and didemnin B (DB) may be found to be safer and more efficacious agents for transplantation rejection and autoimmune disease than CsA, and the importance and relevance of immunophilins needs to be delineated, but they may have a role in immunological signal transduction.
Book ChapterDOI
Bioactive marine peptides: nutraceutical value and novel approaches.
Anupam Giri,Toshiaki Ohshima +1 more
TL;DR: This chapter provides an overview of bioactive peptides derived from marine organisms as well as information about their biological properties and mechanisms of action with potential applications in different areas.
Journal ArticleDOI
Solution Structure of [Me‐L‐Leu7]Didemnin B Determined by NMR Spectroscopy and Refined by MD Calculation
TL;DR: In this article, homo-and heteronuclear two-dimensional NMR techniques were used to assign all H- and C-resonances of the two conformers A and B of [7-(N-methyl-L-leucine)]didemnin B.
References
More filters
Book
NMR of proteins and nucleic acids
TL;DR: The NMR Assignment Problem in Biopolymers, two-Dimensional NMR With Proteins and Nucleic Acids, and Sequence-Specific Resonance Assignments.
Journal ArticleDOI
Investigation of exchange processes by two‐dimensional NMR spectroscopy
TL;DR: In this paper, a general technique for the investigation of exchange processes in molecular systems is proposed and demonstrated and applications include the study of chemical exchange, of magnetization transfer by inter-and intramolecular relaxation in liquids, and of spin diffusion and cross-relaxation processes in solids.
Journal ArticleDOI
Two‐dimensional spectroscopy. Application to nuclear magnetic resonance
TL;DR: In this article, the possibilities for the extension of spectroscopy to two dimensions are discussed, including the elucidation of energy level diagrams, the observation of multiple quantum transitions, and the recording of high-resolution spectra in inhomogenous magnetic fields.