Betaine improves the PCR amplification of GC-rich DNA sequences.
Reads0
Chats0
TLDR
Betaine improves the co-amplification of the two alternatively spliced variants of the prostate-specific membrane antigen mRNA as well as the amplification of the coding cDNA region of c-jun.Abstract:
Betaine improves the co-amplification of the two alternatively spliced variants of the prostate-specific membrane antigen mRNA as well as the amplification of the coding cDNA region of c-jun. It is suggested that betaine improves the amplification of these genes by reducing the formation of secondary structure caused by GC-rich regions and, therefore, may be generally applicable to ameliorate the amplification of GC-rich DNA sequences.read more
Citations
More filters
Journal ArticleDOI
Isothermal Amplification of Nucleic Acids
TL;DR: This review provides a comprehensive overview of the isothermal amplification of nucleic acids encompassing work published in the past two decades including applications in bioanalysis, diagnostics, nanotechnology, materials science, and device integration.
Journal ArticleDOI
Droplet microfluidics for high-throughput biological assays
TL;DR: Droplets allow sample volumes to be significantly reduced, leading to concomitant reductions in cost, and compartmentalization in droplets increases assay sensitivity by increasing the effective concentration of rare species and decreasing the time required to reach detection thresholds.
Journal ArticleDOI
Diversity, distribution and roles of osmoprotective compounds accumulated in halophytes under abiotic stress
TL;DR: The diversity of osmolytes among halophytes and their distribution within taxonomic groups, the intrinsic and extrinsic factors that influence their accumulation, and their role in osmoregulation and osmoprotection are discussed.
Journal ArticleDOI
A moderately thermophilic ammonia-oxidizing crenarchaeote from a hot spring.
Roland Hatzenpichler,Elena V. Lebedeva,Eva Spieck,Kilian Stoecker,Andreas Richter,Holger Daims,Michael Wagner +6 more
TL;DR: The enriched AOA, which is provisionally classified as “Candidatus Nitrososphaera gargensis,” is the first described thermophilic ammonia oxidizer and the first member of the crenarchaeotal group I.1b for which ammonium oxidation has been verified on a cellular level.
References
More filters
Journal ArticleDOI
PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates
TL;DR: The base-pair fidelity, the ability to use PCR products as primers, and the maximum yield of target fragment were increased and improvements were achieved by the combination of an exonuclease-free, N-terminal deletion mutant of Taq DNA polymerase, Klentaq1.
Journal ArticleDOI
Betaine can eliminate the base pair composition dependence of DNA melting
TL;DR: It is shown that the amino acid analogue betaine shares with small tetraalkylammonium ions the ability to reduce or even eliminate the base pair composition dependence of DNA thermal melting transitions, which allows the experimental separation of compositional and polyelectrolyte effects on DNA melting.
Journal ArticleDOI
Formamide can dramatically improve the specificity of PCR.
Journal Article
Alternatively Spliced Variants of Prostate-specific Membrane Antigen RNA: Ratio of Expression as a Potential Measurement of Progression
TL;DR: A tumor index is compiled which reflects the increased expression of PSM over PSM' following the progression from normal to tumor state, and may be a useful indicator for the measurement of tumor progression.
Journal ArticleDOI
Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content.
TL;DR: A PCR method for uniform amplification of a mixture of DNA templates differing in GC content is described using the two enzyme approach (Klentaq1 and Pfu DNA polymerase) and a combination of DMSO and betaine.