Biocompatibility natural effect of rhamnolipids in bioremediation process on different biological systems at the site of contamination

TLDR: The in vitro and in vivo toxicity studies on the mouse fibroblast cell line and rabbit skin surface respectively showed that the crude rhamnolipid mixture exhibits a nontoxic behavior, and the antimicrobial property with respect to bacteria was found to be more prominent toward Gram positive than towards Gram negative.

Abstract: Rhamnolipid is known for its potential for degradation of various harmful and toxic contaminants. However, there is a lack of reports of their effects on different biological systems. Hence, the present study focuses on the assessment of rhamnolipid mixture for their biocompatibility nature. The in vitro and in vivo toxicity studies on the mouse fibroblast cell line (L929) and rabbit skin surface respectively showed that the crude rhamnolipid mixture exhibits a nontoxic behavior (p < 0.05). Similar results were observed with Aedesalbo pictus larvae. However, studies on monocotyledonous and dicotyledonous seeds showed the toxic behavior of rhamnolipids above their critical micelle concentration values (5–200 mg.L − 1). The antimicrobial property with respect to bacteria was found to be more prominent toward Gram positive (0.5 ± 0.5 mg.L − 1) compared to Gram negative bacteria (2.0 ± 0.7 mg.L − 1) and it was least in case of fungi (2.0 ± 0.2 mg.L − 1). The present study opens up a possibility to stu...