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Journal ArticleDOI

Biodegradation of cypermethrin by Micrococcus sp. strain CPN 1.

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TLDR
A bacterium capable of utilizing pyrethroid pesticide cypermethrin as sole source of carbon was isolated from soil and identified as a Micrococcus sp.
Abstract
A bacterium capable of utilizing pyrethroid pesticide cypermethrin as sole source of carbon was isolated from soil and identified as a Micrococcus sp. The organism also utilized fenvalerate, deltamethrin, perimethrin, 3-phenoxybenzoate, phenol, protocatechuate and catechol as growth substrates. The organism degraded cypermethrin by hydrolysis of ester linkage to yield 3-phenoxybenzoate, leading to loss of its insecticidal activity. 3-Phenoxybenzoate was further metabolized by diphenyl ether cleavage to yield protocatechuate and phenol as evidenced by isolation and identification of metabolites and enzyme activities in the cell-free extracts. Protocatechuate and phenol were oxidized by ortho-cleavage pathway. Thus, the organism was versatile in detoxification and complete mineralization of pyrethroid cypermethrin

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Citations
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Journal ArticleDOI

Bioaugmentation of chlorothalonil-contaminated soil with hydrolytically or reductively dehalogenating strain and its effect on soil microbial community.

TL;DR: In insights into the effects of halogenated fungicide application and bioaugmentation on indigenous soil microbiomes, little influence on soil microbial community was observed for each inoculation treatment, showing that TPN treatment is the main force for the shift in indigenous consortia.
Journal ArticleDOI

Cometabolic biotransformation of fenpropathrin by Clostridium species strain ZP3

TL;DR: A novel bacterial strain capable of degrading the pyrethroid pesticide fenpropathrin was isolated from mixed wastewater and sludge samples andPhylogenetic analysis of the 16S rDNA sequence revealed that the organism belongs to the genus Clostridium.
Journal ArticleDOI

Isolation, identification and cyfluthrin-degrading potential of a novel Lysinibacillus sphaericus strain, FLQ-11-1.

TL;DR: The data showed that cyfluthrin could be efficiently degraded by FLQ-11-1, indicating that this strain could potentially be used to eliminate the contamination of pyrethroid herbicides.
Journal ArticleDOI

Stability of the pyrethroid pesticide bifenthrin in milled wheat during thermal processing, yeast and lactic acid fermentation, and storage.

TL;DR: Possible bifenthrin contamination in wheat, in amounts over the maximum residue limits, could not be reduced by sterilisation or by yeast fermentation, but lactic acid fermentation could be an effective tool for minimising residual contamination.
Journal ArticleDOI

Stereoselective Degradation and Molecular Ecological Mechanism of Chiral Pesticides Beta-Cypermethrin in Soils with Different pH Values.

TL;DR: This work has successfully devised a method to investigate the molecular biological mechanisms of the selective degradation behavior of chiral compounds, thus enabling us to better understand these mechanisms.
References
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Journal Article

Protein Measurement with the Folin Phenol Reagent

TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI

A procedure for the isolation of deoxyribonucleic acid from micro-organisms

TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
Book ChapterDOI

[109] Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA

Manley Mandel, +1 more
TL;DR: The GC content of highly purified DNA samples can be estimated from their thermal denaturation temperatures, employing small amounts of DNA, and a recent interesting observation is that the supercoiled component of polyoma DNA has a Tm which is 17° higher than the circular, nonsupercoiled or linear polyoma components.
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