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Biodegradation of cypermethrin by Micrococcus sp. strain CPN 1.

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TLDR
A bacterium capable of utilizing pyrethroid pesticide cypermethrin as sole source of carbon was isolated from soil and identified as a Micrococcus sp.
Abstract
A bacterium capable of utilizing pyrethroid pesticide cypermethrin as sole source of carbon was isolated from soil and identified as a Micrococcus sp. The organism also utilized fenvalerate, deltamethrin, perimethrin, 3-phenoxybenzoate, phenol, protocatechuate and catechol as growth substrates. The organism degraded cypermethrin by hydrolysis of ester linkage to yield 3-phenoxybenzoate, leading to loss of its insecticidal activity. 3-Phenoxybenzoate was further metabolized by diphenyl ether cleavage to yield protocatechuate and phenol as evidenced by isolation and identification of metabolites and enzyme activities in the cell-free extracts. Protocatechuate and phenol were oxidized by ortho-cleavage pathway. Thus, the organism was versatile in detoxification and complete mineralization of pyrethroid cypermethrin

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Journal ArticleDOI

Screening and Genome Sequencing of Deltamethrin-Degrading Bacterium ZJ6

TL;DR: It was found that ZJ6 has relatively strong deltamethrin degradation ability and high value as a subject for further research, and provides a reference to guide the preparation of pesticide-degrading bacterial agents and environmental remediation.
Journal ArticleDOI

Biodegradation competence of Streptomyces toxytricini D2 isolated from leaves surface of the hybrid cotton crop against β cypermethrin.

TL;DR: This is the first report, revealed that the S. toxytricini D2 belongs to the Actinobacteria has the potential to degrade the β cypermethrin into less or nontoxic metabolites under optimized conditions.
Journal ArticleDOI

The Midgut Microbiota of Colombian Aedes aegypti Populations with Different Levels of Resistance to the Insecticide Lambda-cyhalothrin.

TL;DR: It is proposed that kdr mutations are important in the development of resistance to lambda-cyhalothrin at low insecticide concentrations but insect symbionts could play an essential role in the metabolization of pyrethroid insecticides at higher concentrations, contributing to the resistant phenotype in Ae.
Journal ArticleDOI

Advances and future prospects of pyrethroids: Toxicity and microbial degradation.

TL;DR: In this paper , an attempt has been made to analyse the advances and prospects in using pyrethrins and possible technologies to control their harmful effects, along with the impact of pyrethroids on different plants and soil microbial flora.
References
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Journal Article

Protein Measurement with the Folin Phenol Reagent

TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI

A procedure for the isolation of deoxyribonucleic acid from micro-organisms

TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
Book ChapterDOI

[109] Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA

Manley Mandel, +1 more
TL;DR: The GC content of highly purified DNA samples can be estimated from their thermal denaturation temperatures, employing small amounts of DNA, and a recent interesting observation is that the supercoiled component of polyoma DNA has a Tm which is 17° higher than the circular, nonsupercoiled or linear polyoma components.
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