Biosynthesis of thyroglobulin: Partial characterization of a labeled 6 S precursor.
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The slowest sedimenting component was obtained by labeling thyroid glands in vivo or in vitro with radioactive L-leucine and D-mannose and has been purified and further characterized and shown to partially associate in vitroWith half molecules of thyroglobulin (12 S subunits) and to react with antibodies against rat thyrogLobulin.About:
This article is published in FEBS Letters.The article was published on 1969-08-01 and is currently open access. It has received 14 citations till now. The article focuses on the topics: Thyroglobulin.read more
Citations
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Book ChapterDOI
Chemistry and Biosynthesis of Thyroid Iodoproteins
G. Salvatore,H. Edelhoch +1 more
TL;DR: This chapter discusses the chemistry and the biosynthesis of thyroid iodoproteins, which represent not only the form in which thyroid hormones are stored but also the molecular site of hormonal biosynthesis.
Journal ArticleDOI
Properties of thyroglobulin. XX. The biosynthesis of thyroglobulin. Effect of temperature on subunit species.
TL;DR: Iodine labeling follows a completely different pattern from carbon labeling of thyroglobulin, and this result is in harmony with iodine incorporation occurring after and independent of protein synthesis.
Journal ArticleDOI
In vitro synthesis of 300 oo Mr rat thyroglobulin subunit
TL;DR: The 300 000 Mr polypeptide specified by this RNA has been identified as thyroglobulin on the basis of the following criteria: (i) Immunoprecipitation by purified, monospecific anti-thyrogLobulin antibody;
Journal ArticleDOI
Identification and Characterization of Two Labeled Intermediates in the Biosynthesis of Rat Thyroglobulin
TL;DR: It is concluded that the 6 S and 7 S labeled proteins participate in the structure of newly formed unstable 19 S molecules and may represent intermediates during the assembly process of the elementary polypeptide chains of thyroglobulin.
Journal ArticleDOI
Synthesis and association of thyroglobulin subunits in a polyribosomal cell-free system
TL;DR: Thyroglobulin synthesis was investigated in a polyribosomal cell-free system prepared from bovine thyroid glands and was partially resistant to 6 M guanidine, indicating that covalent bonds were involved in the incorporation of subunits into thyroglOBulin.
References
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Journal ArticleDOI
The properties of thyroglobulin. I. The effects of alkali.
TL;DR: With thyroglobulin extracted from calf thyroid tissue, a new component, which possessed quite similar sedimentation properties to that reported by Lundgren and Williams, was observed to form at higher ionic strengths when the pH was raised above the neutral range.
Journal ArticleDOI
Separation of Thyroidal Iodoproteins and Purification of Thyroglobulin by Gel Filtration and Density Gradient Centrifugation
TL;DR: Filtration through granulated agar or dextran gel columns was used for the purification of thyroglobulin; ultracentrifugation in a linear density gradient was employed for both analytical and preparative purposes.
Journal ArticleDOI
Glycoprotein Biosynthesis: Studies on Thyroglobulin CHARACTERIZATION OF A PARTICULATE PRECURSOR AND RADIOISOTOPE INCORPORATION BY THYROID SLICES AND PARTICLE SYSTEMS
Robert G. Spiro,Mary Jane Spiro +1 more
TL;DR: Special precautions are called to be taken in the performance of ribonucleic acid determinations in the thyroid in order to avoid the overestimation of this nucleic acid which may result from the large amount of carbohydrate present in this tissue in the form of thyroglobulin.