Journal ArticleDOI
Capacitation and acrosome reactions in human spermatozoa monitored by a chlortetracycline fluorescence assay
Michael A. Lee,Giuliana S. Trucco,Kathleen B. Bechtol,Neil Wummer,Gregory S. Kopf,Luis Blasco,Bayard T. Storey +6 more
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TLDR
In this paper, human spermatozoa were incubated in culture medium containing human serum albumin (HSA) to promote capacitation, which was monitored by a rapid chlortetracycline (CTC) fluorescence assay.About:
This article is published in Fertility and Sterility.The article was published on 1987-10-01. It has received 180 citations till now. The article focuses on the topics: Acrosome reaction & Acrosome.read more
Citations
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Journal ArticleDOI
Dynamics of the mammalian sperm plasma membrane in the process of fertilization.
TL;DR: Dynamics in adhesive and fusion properties, molecular composition and architecture of the sperm plasma membrane, as well as membrane derived signalling are reviewed.
Journal ArticleDOI
Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa
Juan G. Alvarez,Bayard T. Storey +1 more
TL;DR: Intact human sperm incorporated radiolabelled fatty acids into membrane phospholipids when incubated in medium containing bovine serum albumin as a fatty acid carrier, indicating that aospholipase A2 may act in peroxidative defense by excising a hydroperoxy acyl group from phospholIPid and providing the hydro peroxy fatty acid product as substrate to glutathione peroxIDase.
Journal ArticleDOI
The molecular basis of sperm capacitation.
Pablo E. Visconti,Hannah Galantino-Homer,Grace D. Moore,Janice L. Bailey,XiaoPing Ning,Miguel W. Fornés,Gregory S. Kopf +6 more
TL;DR: A brief review will consider some of the recent findings made toward an understanding of capacitation using in vitro models and offers an offer of an exhaustive analysis of this important event.
Journal ArticleDOI
An adhesion-associated agonist from the zona pellucida activates G protein-promoted elevations of internal Ca2+ and pH that mediate mammalian sperm acrosomal exocytosis.
TL;DR: Results indicate that a developmentally regulated mechanism of signal transduction employs G protein(s) to couple the physiological (zona) agonist to alterations of the internal ionic mediators of acrosomal exocytosis.
Journal ArticleDOI
N-Acylethanolamines in human reproductive fluids.
Herbert Schuel,Lani J. Burkman,Jack Lippes,Kent Crickard,Ellyn Forester,Daniele Piomelli,Andrea Giuffrida +6 more
TL;DR: Analysis by high-performance liquid chromatography/mass spectrometry shows the presence of AEA, PEA, and OEA in human seminal plasma, mid-cycle oviductal fluid, follicular fluid, amniotic fluid, milk, and fluids from malignant ovarian cysts, suggesting that NAEs in human reproductive fluids may help regulate multiple physiological and pathological processes in the reproductive system and imply that exogenous cannabinoids delivered by marijuana smoke might impact these processes.
References
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Journal ArticleDOI
The Use of Zona-Free Animal Ova as a Test-System for the Assessment of the Fertilizing Capacity of Human Spermatozoa
TL;DR: The results suggest that zona-free hamster ova can be substituted for human ova in the preliminary assessment of the fertilizing capacity of human spermatozoa.
Book ChapterDOI
Mechanisms of Fertilization in Mammals
TL;DR: This chapter will discuss how mammalian spermatozoa prepare themselves for fertilization and how the spermatozosa and eggs interact during fertilization.
Journal ArticleDOI
The capacitation of the mammalian sperm.
TL;DR: It is shown that sperms, injected into the Fallopian tubes after ovulation, were able to penetrate a larger proportion of eggs if they had first spent about five hours in the uterus of another rabbit.
Journal ArticleDOI
Sperm-egg interactions in the mouse: Sequence of events and induction of the acrosome reaction by a zona pellucida glycoprotein☆
TL;DR: It is suggested that, at least in vitro, mouse sperm recognize and bind to ZP3 of egg zonae pellucidae, and that such binding leads to the induction of the acrosome reaction.
Related Papers (5)
Determination of the time course of capacitation in mouse spermatozoa using a chlortetracycline fluorescence assay.
Cynthia R. Ward,Bayard T. Storey +1 more