Catalysis of Both Oxidation and Reduction of Glyoxylate by Pig Heart Lactate Dehydrogenase Isozyme 1
Reads0
Chats0
TLDR
Pig heart l-lactate dehydrogenase isozyme 1 was purified by preparative polyacrylamide gel electrophoresis and the pattern of inhibition by oxalate and oxamate is similar to that observed when pyruvate and lactate are used as substrates for the enzyme.About:
This article is published in Journal of Biological Chemistry.The article was published on 1970-04-10 and is currently open access. It has received 72 citations till now. The article focuses on the topics: Glyoxylate cycle & Lactate dehydrogenase.read more
Citations
More filters
Book ChapterDOI
4 Lactate Dehydrogenase
TL;DR: This chapter discusses the lactate dehydrogenase, the only simpler dehydrogen enzyme where both structure and sequence are known at present.
Journal ArticleDOI
Role of the methylcitrate cycle in Mycobacterium tuberculosis metabolism, intracellular growth, and virulence
TL;DR: The role of the methylcitrate cycle in M. tuberculosis metabolism was further evaluated by constructing a mutant strain in which prpC (encoding MCS) and prpD (decoding MCD) were deleted as discussed by the authors, and the mutant strain could not grow on propionate media in vitro or in murine bone marrow-derived macrophages infected ex vivo.
Journal ArticleDOI
Characterization of Activity and Expression of Isocitrate Lyase in Mycobacterium avium and Mycobacterium tuberculosis
TL;DR: Express and purify Icl, as well as AceA proteins, and show that both exhibit isocitrate lyase activity, and it is presented evidence that in both M. avium and M. tuberculosis the production and activity of the isOCitrate Lyase is enhanced under minimal growth conditions when supplemented with acetate or palmitate.
Journal ArticleDOI
RamB, a novel transcriptional regulator of genes involved in acetate metabolism of Corynebacterium glutamicum
TL;DR: Results indicate that RamB is a negative transcriptional regulator of genes involved in acetate metabolism of C. glutamicum, and this deregulation takes place at the level of transcription.
Journal ArticleDOI
Characterization of the isocitrate lyase gene from Corynebacterium glutamicum and biochemical analysis of the enzyme.
TL;DR: The native enzyme was shown to be a tetramer of identical subunits, to exhibit an ordered Uni-Bi mechanism of catalysis, and to be effectively inhibited by 3-phosphoglycerate, 6-ph phosphogluconate, phosphoenolpyruvate, fructose-1,6-bisphosphate, and succinate.
References
More filters
Journal ArticleDOI
Disc electrophoresis – ii method and application to human serum proteins*
TL;DR: The technique of disc electrophoresis has been presented, including a discussion of the technical variables with special reference to the separation of protein fractions of normal human serum.
Journal ArticleDOI
The Determination of Enzyme Dissociation Constants
Hans Lineweaver,Dean Burk +1 more
TL;DR: On the basis of the assumed theory the rate of the observed reaction is directly proportional to the concentration of the enzyme-substrate compound, where (E:l = (ES).
Journal ArticleDOI
The oxidation of ribonuclease with performic acid.
TL;DR: The present investigation was undertaken to determine quantitatively the stability of each of the amino acid residues in the ribonuclease molecule under the conditions employed for the oxidation of the cystine sulfur bridges.
Journal ArticleDOI
A procedure for rapid and sensitive staining of protein fractionated by polyacrylamide gel electrophoresis
TL;DR: Protein zones may be stained by Coomassie blue without any destaining of the background and with a sensitivity at least as high as that of amido black and at the price of background destaining.
Related Papers (5)
L-glyceric aciduria. A new genetic variant of primary hyperoxaluria.
Hyperoxaluria in L-glyceric aciduria: possible pathogenic mechanism.
Isolation and characterization of glycolic acid dehydrogenase from human liver
David W. Fry,K.E. Richardson +1 more