Journal ArticleDOI
Cell biology of the primitive eukaryote Giardia lamblia.
Reads0
Chats0
TLDR
It is found that G. lamblia forms novel encystation-specific secretory vesicles and can sort cyst wall proteins to a regulated secretory pathway distinct from the constitutive pathway used to transport the variable cysteine-rich protein to the trophozoite surface.Abstract:
Giardia lamblia is an extremely primitive or early-diverging eukaryote that has been considered to have no typical ER or Golgi apparatus, although it is a complex and highly developed cell. Both the trophozoite and cyst have unusual surface proteins that enable these stages to survive in very different and hostile environments. We found that G. lamblia forms novel encystation-specific secretory vesicles and can sort cyst wall proteins to a regulated secretory pathway distinct from the constitutive pathway used to transport the variable cysteine-rich protein to the trophozoite surface. Our studies, utilizing novel ultrastructural methods that preserve the endomembranes, as well as IEM, support the idea that G. lamblia has many of the endomembrane protein transport elements and sorting functions of higher cells and that these appeared very early in the evolution of eukaryotic cells.read more
Citations
More filters
Journal ArticleDOI
Biology of Giardia lamblia.
TL;DR: The Giardia genome project promises to greatly increase the understanding of this interesting and enigmatic organism.
Journal ArticleDOI
Drug targets and mechanisms of resistance in the anaerobic protozoa
TL;DR: The review examines the value of investigating both clinical and laboratory-induced syngeneic drug-resistant isolates and dissection of the complementary data obtained, as well as thevalue of studies of the epidemiology of resistance.
Journal ArticleDOI
Golgi Architecture and Inheritance
James Shorter,Graham Warren +1 more
TL;DR: This review attempts to integrate recent findings to depict a more coherent, synthetic molecular picture of mitotic Golgi fragmentation and reassembly and considers the emerging concept of a highly regulated and dynamic Golgi structural matrix or template that interfaces with cargo receptors, Golgi enzymes, Rab-GTPases, and SNAREs to tightly couple biosynthetic transport to Golgi architecture.
Journal ArticleDOI
The origin of the eukaryotic cell: a genomic investigation.
Hyman Hartman,Alexei Fedorov +1 more
TL;DR: The eukaryotic signature proteins (ESPs) as mentioned in this paper are a set of 347 proteins that are found in eukarial cells but have no significant homology to proteins in Archaea and Bacteria.
Journal ArticleDOI
Genome ploidy in different stages of the Giardia lamblia life cycle.
TL;DR: The nuclear and cellular genome ploidy of G. lamblia cells during all stages of the life cycle is determined and it is suggested that the newly excysted cell, for which the term ‘excyzoite’ is suggested, contains four nuclei.
References
More filters
Journal ArticleDOI
Protein disulphide isomerase: building bridges in protein folding
TL;DR: Recent mutagenesis studies are helping to unravel the catalytic mechanism of PDI, and work in yeast and other systems is clarifying the physiological roles of the multiple PDI-related proteins.
Journal ArticleDOI
Phylogenetic meaning of the kingdom concept: an unusual ribosomal RNA from Giardia lamblia
TL;DR: Investigation of the small subunit ribosomal RNA (16S-like rRNA) from the protozoan Giardia lamblia provided a new perspective on the evolution of nucleated cells and challenged the phylogenetic significance of multiple eukaryotic kingdoms.
Journal ArticleDOI
Progress in unraveling pathways of golgi traffic
TL;DR: Flow of Biosynthetic Traffic to and through the Golgi Complex and N-Glycosylation Processing Steps used to Determine Compartmentation Along the Secretory Pathway are studied.
Journal ArticleDOI