Changes in Microbiota Across Developmental Stages of Aedes koreicus, an Invasive Mosquito Vector in Europe: Indications for Microbiota-Based Control Strategies.
Niccolò Alfano,Valentina Tagliapietra,Fausta Rosso,Mattia Manica,Daniele Arnoldi,Massimo Pindo,Annapaola Rizzoli +6 more
TLDR
The results suggest that the microbiota of Ae.Abstract:
Since it has been understood that gut microbiota of vector mosquitoes can influence their vector competence, efforts have been undertaken to develop new control strategies based on host microbiota manipulation, and aimed at suppressing the vector population or replacing it with a less competent one. For the proper design of such control strategies it is necessary to know the microbiota composition of the target vector species, how it is acquired, and how it changes throughout the host’s life cycle. In this study, 16S rRNA amplicon high-throughput sequencing was used to characterize the changes in microbiota from the aquatic environment (larval breeding sites) to the different developmental stages of field-collected Aedes koreicus in Italy, an emerging invasive mosquito species in Europe and a potential vector of several pathogens. The bacterial communities of the aquatic breeding sites, larvae, pupae and adults showed distinctive structures to one another. Indeed, 84% of community members were unique to a given sample type. Nevertheless, almost 40% of the sequences generated were assigned to bacteria detected in all sample types, suggesting the importance of bacteria transstadially transmitted from water to the adult stage in constituting mosquito microbiota. Among these, genus C39 largely constituted water microbiota, family Burkholderiaceae was the most abundant in larvae and pupae, and genus Asaia dominated adult communities. In addition, Asaia consitituted a core microbiota across all sample types. Our results suggest that the microbiota of Ae. koreicus mosquitoes is composed by a community which derives from the aquatic bacteria of the larval breeding sites, is then filtered by the larval gut, where only certain members are able to persist, rearranged by metamorphosis and finally modified by the change in diet at the adult stage. Understanding how the microbiota of Ae. koreicus changes through the mosquito life cycle represents a first step in selecting bacterial candidates for use in microbiota-based intervention measures for this species. The properties which Asaia exhibits in this species, such as dominance, high prevalence and transstadial transmission, prevent the use of Wolbachia but make Asaia an ideal candidate for paratransgenesis.read more
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Microbiome Composition and Microbial Community Structure in Mosquito Vectors Aedes aegypti and Aedes albopictus in Northeastern Thailand, a Dengue-Endemic Area
Rutchanee Rodpai,Patcharaporn Boonroumkaew,Lakkhana Sadaow,Oranuch Sanpool,Penchom Janwan,Tongjit Thanchomnang,Pewpan M. Intapan,Wanchai Maleewong +7 more
TL;DR: In this paper , the authors characterized the microbiota associated with Aedes aegypti and Aedes albopictus larvae and subsequently emerged adults relative to the microbiota in water from their breeding sites in Thailand, a dengue-endemic area.
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Aedes albopictus microbiome derives from environmental sources and partitions across distinct host tissues
TL;DR: In this article , the authors use ecological network analysis to examine how environmental bacteria assemble to form bacteriomes among Aedes albopictus host tissues, and find that the bacteriome of A. albopsictus tissues were compositional taxonomic subsets of environmental bacterioms and suggest that the environmental microbiome serves as a source pool that supports mosquito microbiome diversity.
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Marked Effects of Larval Salt Exposure on the Life History and Gut Microbiota of the Malaria Vector Anopheles merus (Diptera: Culicidae)
Ashmika Singh,Nashrin F. Patel,Mushal Allam,Wai-Yin Chan,Thabo Mohale,Arshad Ismail,Shüné V. Oliver +6 more
TL;DR: In this article , the authors examined the effects of exposure to 0, 2.1875, 4.375, 8.75, and 17.5 g/L of sodium chloride on laboratory-bred An. merus.
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Climate Change and Transmissible Diseases
TL;DR: In this article , the authors present a state-of-the-art of the interactions of climate change effects on transmissible diseases based on authors previous experience and knowledge on the subjects hereby addressed.
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Three Species of Axenic Mosquito Larvae Recruit a Shared Core of Bacteria in a Common Garden Experiment
TL;DR: In this paper , the authors describe the generation of two new species of axenic mosquito, Aedes albopictus and Aedes triseriatus, which were reared either individually or combinatorially with the other species to characterize the effects of co-rearing on the composition of the microbiome.
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