Journal ArticleDOI
Channel-resolved multianalyte immunosensing system for flow-through chemiluminescent detection of α-fetoprotein and carcinoembryonic antigen
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TLDR
This novel immunosensing system based on the designed channel-resolved technique provided an automated, reusable, simple, sensitive and low-cost approach for multianalyte immunoassay without using of expensive array detector.About:
This article is published in Biosensors and Bioelectronics.The article was published on 2008-02-28. It has received 78 citations till now. The article focuses on the topics: Immunoassay.read more
Citations
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Sensitive Immunosensor for Cancer Biomarker Based on Dual Signal Amplification Strategy of Graphene Sheets and Multienzyme Functionalized Carbon Nanospheres
Dan Du,Zhexiang Zou,Yongsoon Shin,Jun Wang,Hong Wu,Mark H. Engelhard,Jun Liu,Ilhan A. Aksay,Yuehe Lin +8 more
TL;DR: A novel electrochemical immunosensor for sensitive detection of cancer biomarker alpha-fetoprotein (AFP) is described that uses a graphene sheet sensor platform and functionalized carbon nanospheres labeled with horseradish peroxidase-secondary antibodies (HRP-Ab2) based on a dual signal amplification strategy.
Journal ArticleDOI
Enzyme-functionalized silica nanoparticles as sensitive labels in biosensing.
TL;DR: This strategy was successfully demonstrated as a simple, cost-effective, specific, and potent method to detect AFP in practical samples and possessed high sensitivity and reproducibility.
Journal ArticleDOI
Ultrasensitive electrochemical immunosensor for PSA biomarker detection in prostate cancer cells using gold nanoparticles/PAMAM dendrimer loaded with enzyme linked aptamer as integrated triple signal amplification strategy.
TL;DR: The obtained results indicate the proposed immunosensor can be used for monitor the differences in PSA concentration in cancer tissue samples which holds great promise in clinical screening of cancer biomarkers.
Journal ArticleDOI
Amplified Electrochemical Detection of a Cancer Biomarker by Enhanced Precipitation Using Horseradish Peroxidase Attached on Carbon Nanotubes
TL;DR: An electrochemical nanoimmunosensor based on multiwall carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) was developed for the amplified detection of prostate specific antigen (PSA) and the validity of the proposed method was compared with an enzyme-linked immunosorbent assay method.
Journal ArticleDOI
Gold nanolabels for new enhanced chemiluminescence immunoassay of alpha-fetoprotein based on magnetic beads.
TL;DR: The present method was successfully applied to the determination of AFP in human serum samples and indicated that this proposed protocol could be quite promising for the application in immunoassays.
References
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Journal ArticleDOI
Multiplexed toxin analysis using four colors of quantum dot fluororeagents.
Ellen R. Goldman,Aaron R. Clapp,George M. Anderson,H. Tetsuo Uyeda,J. Matthew Mauro,Igor L. Medintz,Hedi Mattoussi +6 more
TL;DR: This work prepared bioinorganic conjugates made with highly luminescent semiconductor nanocrystals and antibodies and antibodies to perform multiplexed fluoroimmunoassays and demonstrated the simultaneous detection of the four toxins from a single sample probed with a mixture of all four QD-antibody reagents.
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Determination of carcinoembryonic antigen in human sera by integrated bead-bed immunoasay in a microchip for cancer diagnosis
TL;DR: This microchip-based diagnosis system is the first micro chip-based system that is practically useful for clinical diagnoses with short analysis time, high sensitivity, and easy procedures.
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Microfluidic Enzyme Immunoassay Using Silicon Microchip with Immobilized Antibodies and Chemiluminescence Detection
Julia Yakovleva,Richard Davidsson,Anna Lobanova,Martin Bengtsson,Sergei A. Eremin,Thomas Laurell,Jenny Emnéus +6 more
TL;DR: The buffer composition was shown to have a dramatic effect on the assay stability, where the commonly used phosphate buffer saline was proved to be the least suitable choice.
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Electrochemical Coding for Multiplexed Immunoassays of Proteins
TL;DR: Electrochemical coding could be readily multiplexed and scaled up in multiwell microtiter plates to allow simultaneous parallel detection of numerous proteins or samples and is expected to open new opportunities for protein diagnostics and biosecurity.
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Automated microarray system for the simultaneous detection of antibiotics in milk.
Bertram G. Knecht,Angelika Strasser,Richard Dietrich,Erwin Märtlbauer,Reinhard Niessner,Michael G. Weller +5 more
TL;DR: The PASA system proved to be the first immunochemical biosensor platform having the potential to test for numerous antibiotics in parallel, such being of considerable interest for the control of milk in the dairy industry.