Characterization of North American Armillaria species by nuclear DNA content and RFLP analysis
Mee-Sook Kim,Ned B. Klopfenstein,Geral I. McDonald,Kathiravetpillai Arumuganathan,Anne K. Vidaver +4 more
TLDR
Polymerase chain reaction (PCR) and RFLP of the intergenic spacer region-1 (IGS-1) generated banding patterns for nine Armillaria species, and new banded patterns are presented for A. ostoyae, A. gemina, and A. mellea.Abstract:
Twenty-six isolates representing nine North American Armillaria species were investigated with flow cytometry and RFLP (restriction fragment length polymorphism) analyses to determine their nuclear DNA content and RFLP profile. Three pu- tatively diploid isolates of A. ostoyae, A. gemina, A. calvescens, A. sinapina, A. mellea, A. gallica, A. nabs- nona, and North American Biological Species (NABS) X were analyzed, and two putatively diploid isolates of NABS XI also were analyzed. Nuclear DNA contents of Armillaria species were 0.11-0.17 pg per nucleus (55-84 X 10" bp/C), depending on species. Among the nine North American Armillaria species tested, A. ostoyae, A. gemirza, and A. mellea possessed relatively small nuclear DNA contents (0.1 1-0.12 pg per nucleus), whereas A. gallica possessed a relatively large nuclear DNA content (0.17 pg per nucleus). A. nabsno,na has a slightly larger nuclear DNA content (0.13pg per nucleus) than A. ostojae, A. gemina, and A. mellea. Other species (A. calvescens, A. sinapina, NABS X, and NABS XI) possessed moderate nuclear DNA contents (ca 0.15 pg per nucleus). Polymerase chain reaction (PCR) and RFLP of the intergenic spacer region-1 (IGS-1) generated banding patterns for nine Armillaria species. In addition to previously reported banding patterns, new banding patterns areread more
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Coarse-scale population structure of pathogenic Armillaria species in a mixed-conifer forest in the Blue Mountains of northeast Oregon
TL;DR: The coarse-scale population structure of pathogenic Armillaria (Fr.) Staude species was determined on approximately 16 100 ha of relatively dry, mixed-conifer forest in the Blue Mountains of northeast Oregon.
Journal ArticleDOI
Genomic and proteomic dissection of the ubiquitous plant pathogen, Armillaria mellea: toward a new infection model system.
Cassandra Collins,Thomas M. Keane,Daniel J. Turner,Grainne O'Keeffe,David A. Fitzpatrick,Sean Doyle +5 more
TL;DR: The A. mellea genome comprises 58.35 Mb, contains 14473 gene models, and reveals a rich reservoir of carbohydrate degrading enzymes, laccases, and lignin peroxidases in the proteome, reminiscent of both basidiomycete and ascomycete glycodegradative arsenals.
Journal ArticleDOI
Characterization of North American Armillaria species: genetic relationships determined by ribosomal DNA sequences and AFLP markers
TL;DR: The results indicate that AFLP genetic markers offer potential for distinguishing currently recognized North American Biological Species (NABS) of Armillaria in future biological, ecological and taxonomic studies.
Journal ArticleDOI
Dothistroma needle blight, weather and possible climatic triggers for the disease's recent emergence.
Alex J. Woods,Jorge Martín-García,L. S. Bulman,Marta W. Vasconcelos,Johanna Boberg,N. La Porta,H. Peredo,G. Vergara,Rodrigo Ahumada,A. V. Brown,Julio J. Diez +10 more
TL;DR: It is found that since the 1950s, four of the past five strong El Nino events appear to have coincided with reports of increased DNB activity on an intercontinental scale, and the projected future climatic conditions in the Northern Hemisphere appear to be increasingly favourable for the disease.
Journal ArticleDOI
Fungal endophytes in woody roots of Douglas-fir (Pseudotsuga menziesii) and ponderosa pine (Pinus ponderosa)
J. A. Hoff,J. A. Hoff,J. A. Hoff,Ned B. Klopfenstein,Geral I. McDonald,J. R. Tonn,Mee-Sook Kim,Paul J. Zambino,P. F. Hessburg,Jack D. Rogers,Tobin L. Peever,Lori M. Carris +11 more
TL;DR: This study found no significant evidence of host species or plant association effects on total recovery of fungal endophytes or recovery of specific fungal taxa.
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Journal ArticleDOI
Nuclear DNA content of some important plant species
K. Arumuganathan,E. D. Earle +1 more
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David W. Galbraith,Kristi R. Harkins,Joyce M. Maddox,Nicola M. Ayres,Dharam P. Sharma,Ebrahim Firoozabady +5 more
TL;DR: The amount of nuclear DNA in the homogenates of monocotyledonous and dicotylingonous plants was accurately and rapidly determined by flow microfluorometry, and the distribution of nuclei involved in the cell cycle was charted for tissues selected from different physical locations or developmental stages.
Journal ArticleDOI
Interfertility and clonal size in the Armillariella mellea complex
TL;DR: The naturally occurring clones of Armillariella species have been identified by using incompatibility factors as genetic markers and on the basis of demarcation line formation in confrontations between diploid pure cultures in good agreement.
Journal ArticleDOI
Molecular phylogeny of northern hemisphere species of Armillaria
TL;DR: This study used polymerase chain reaction amplification and direct sequencing of double-stranded DNA to compare the intergenic region between the 3' end of the 26S gene and the 5S gene of the nuclear, ribosomal DNA of several northern hemisphere species of Armillaria.
Journal ArticleDOI
The primary and secondary structure of yeast 26S rRNA
Geertruida M. Veldman,Jacobus Klootwijk,V.C.H.F. de Regt,Rudi J. Planta,Christiane Branlant,Alain Krol,Jean-Pierre Ebel +6 more
TL;DR: The sequence of the 26S rRNA of the yeast Saccharomyces carlsbergensis is presented as inferred from the gene sequence; the molecule is 3393 nucleotides long and consists of 48% G+C; 30 of the 43 methyl groups can be located in the sequence.