Chemokines IL-8, GROα, MCP-1, IP-10, and Mig Are Sequentially and Differentially Expressed During Phase-Specific Infiltration of Leukocyte Subsets in Human Wound Healing
Eva Engelhardt,Atiye Toksoy,Matthias Goebeler,Sebastian Debus,Eva-Bettina Bröcker,Reinhard Gillitzer +5 more
TLDR
It is suggested that a dynamic set of chemokines contributes to the spatially and temporally different infiltration of leukocyte subsets and thus integrates the inflammatory and reparative processes during wound repair.Abstract:
Healing of cutaneous wounds requires a complex integrated network of repair mechanisms, including the action of newly recruited leukocytes. Using a skin repair model in adult humans, we investigated the role chemokines play in sequential infiltration of leukocyte subsets during wound healing. At day 1 after injury, the C-X-C chemokines IL-8 and growth-related oncogene α are maximally expressed in the superficial wound bed and are spatially and temporally associated with neutrophil infiltration. IL-8 and growth-related oncogene α profiles also correlate with keratinocyte migration and subsequently subside after wound closure at day 4. Macrophage infiltration reaches the highest levels at day 2 and is paralleled by monocyte chemoattractant protein-1 mRNA expression in both the basal layer of the proliferative epidermis at the wound margins and mononuclear cells in the wound area. Other monocyte-attracting chemokines such as monocyte chemoattractant protein-3, macrophage inflammatory protein-1α and -1β, RANTES, and I309 are undetectable. At day 4, perivascular focal lymphocyte accumulation correlates with strong focal expression of the C-X-C chemokines Mig and IP-10. Our results suggest that a dynamic set of chemokines contributes to the spatially and temporally different infiltration of leukocyte subsets and thus integrates the inflammatory and reparative processes during wound repair.read more
Citations
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Journal ArticleDOI
Regulation of Wound Healing by Growth Factors and Cytokines
Sabine Werner,Richard Grose +1 more
TL;DR: This review summarizes the results of expression studies that have been performed in rodents, pigs, and humans to localize growth factors and their receptors in skin wounds and reports on genetic studies addressing the functions of endogenous growth factors in the wound repair process.
Journal ArticleDOI
Growth factors and cytokines in wound healing.
TL;DR: A review of the specific roles of these growth factors and cytokines during wound healing can be found in this article, where patients are treated by three growth factors: PDGF-BB, bFGF, and GM-CSF.
Journal ArticleDOI
Inflammation in wound repair: molecular and cellular mechanisms.
TL;DR: Cellular and molecular mechanisms controlling inflammation in cutaneous tissue repair are reviewed and a rationale for targeting the inflammatory phase in order to modulate the outcome of the healing response is provided.
Journal ArticleDOI
Inflammation and wound healing: the role of the macrophage.
Timothy J. Koh,Luisa A. DiPietro +1 more
TL;DR: The macrophage continues to be an attractive therapeutic target, both to reduce fibrosis and scarring, and to improve healing of chronic wounds, as a result of advances in the understanding of this multifunctional cell.
Journal ArticleDOI
Chemokines in cutaneous wound healing.
TL;DR: This review will focus preferentially on the role of chemokines duringskin wound healing and intends to provide an update on the multiplefunctions of individual chemokine functions during the phases of woundrepair.
References
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Journal ArticleDOI
Wound Healing--Aiming for Perfect Skin Regeneration
TL;DR: Details of how these signals control wound cell activities are beginning to emerge, and studies of healing in embryos have begun to show how the normal adult repair process might be readjusted to make it less like patching up and more like regeneration.
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Journal ArticleDOI
Interleukin-8 as a Macrophage-Derived Mediator of Angiogenesis
Alisa E. Koch,Peter J. Polverini,Steven L. Kunkel,Lisa A. Harlow,Luisa A. DiPietro,Victor M. Elner,Susan G. Elner,Robert M. Strieter +7 more
TL;DR: A function for macrophage-derived IL-8 in angiogenesis-dependent disorders such as rheumatoid arthritis, tumor growth, and wound repair is suggested.
Journal ArticleDOI
Properties of the Novel Proinflammatory Supergene "Intercrine" Cytokine Family
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The Molecular and Cellular Biology of Wound Repair
Richard A.F. Clark,P. M. Henson +1 more
TL;DR: Wound Repair: Overview and General Considerations (R.A.F. Clark), Macrophage Involvement in Wound Repair, Remodeling and Fibrosis, and the Role of Plateletderived Growth Factor in vivo (C.W. Riches).