Journal ArticleDOI
Clustering of breakpoints on chromosome 11 in human B-cell neoplasms with the t(11;14) chromosome translocation.
Yoshihide Tsujimoto,Elaine S. Jaffe,Jeffrey Cossman,J. Gorham,Peter C. Nowell,Carlo M. Croce +5 more
Reads0
Chats0
TLDR
It is speculated that the t(11 ; 14) chromosome translocation in CLL may be sequence specific and may involve the recombination system for immunoglobulin gene segment (V–D–J) joining.Abstract:
The t(11;14) (q13;q32) chromosome translocation has been reported in diffuse small and large cell lymphomas and in chronic lymphocytic leukaemia (B-CLL) and multiple myeloma. Because chromosome band 14q32 is involved in this translocation, as well as in the t(8;14) (q24;q32) translocation of the Burkitt tumour, interruption of the immunoglobulin heavy-chain locus was postulated for this rearrangement. We have cloned the chromosomal joinings between chromosomes 11 and 14 and also between chromosomes 14 and 18, in B-cell tumours carrying translocations involving these chromosomes, and suggested the existence of two translocated loci, bcl-1 and bcl-2, normally located on chromosomes 11 (band q13) and 18 (band q21) respectively, involved in the pathogenesis of human B-cell neoplasms. The results indicate that in the leukaemic cells from two different cases of CLL, the breakpoints on chromosome 11 are within 8 nucleotides of each other and on chromosome 14 involve the J4-DNA segment. Because we detected a 7mer-9mer signal-like sequence with a 12-base-long spacer on the normal chromosome 11, close to the breakpoint, we speculate that the t(11;14) chromosome translocation in CLL may be sequence specific and may involve the recombination system for immunoglobulin gene segment (V-D-J) joining.read more
Citations
More filters
Journal ArticleDOI
Involvement of the bcl-2 gene in human follicular lymphoma
TL;DR: Chromosome 18-specific DNA probes for the areas flanking the breakpoints also detected RNA transcripts 6 kilobases in length in various cell types, and the gene coding for these transcript seems to be interrupted in most cases of follicular lymphomas carrying the t(14;18) chromosomal translocation.
Journal ArticleDOI
MicroRNA: Biogenesis, Function and Role in Cancer.
TL;DR: The P-body model outlines microRNA sorting and shuttling between specialized P- body compartments that house enzymes required for slicer –dependent and –independent silencing, addressing the reversibility of these silencing mechanisms.
Journal ArticleDOI
Cytochrome C-Mediated Apoptosis
Xuejun Jiang,Xiaodong Wang +1 more
TL;DR: Recent progress in understanding the biochemical mechanisms and regulation of the caspase activation pathway, the roles of the pathway in physiology and disease, and their potential therapeutic values are focused on.
Journal ArticleDOI
Analysis of the structure, transcripts, and protein products of bcl-2, the gene involved in human follicular lymphoma.
TL;DR: It is determined that the bcl-2 (B-cell leukemia/lymphoma 2) gene is transcribed into three overlapping mRNAs, and the cDNA clones corresponding to the three bCl-2 transcripts contain at least two exons.
Journal ArticleDOI
The t(14;18) chromosome translocations involved in B-cell neoplasms result from mistakes in VDJ joining
Yoshihide Tsujimoto,James Gorham,Jeffrey Cossman,Elaine S. Jaffe,Carlo M. Croce,Carlo M. Croce +5 more
TL;DR: It is concluded that the t(14;18) chromosome translocation is the result of a mistake during the process of VDJ joining at the pre-B-cell stage of differentiation.
References
More filters
Book ChapterDOI
Sequencing end-labeled DNA with base-specific chemical cleavages.
Allan M. Maxam,Walter Gilbert +1 more
TL;DR: The chapter presents techniques for producing discrete DNA fragments, end-labeling DNA, segregating end- labeled fragments, extracting DNA from gels, and the protocols for partially cleaving it at specific bases using the chemical reactions.
Journal ArticleDOI
Somatic generation of antibody diversity
TL;DR: In the genome of a germ-line cell, the genetic information for an immunoglobulin polypeptide chain is contained in multiple gene segments scattered along a chromosome which are assembled by recombination which leads to the formation of a complete gene.
Journal ArticleDOI
Cloning of the chromosome breakpoint of neoplastic B cells with the t(14;18) chromosome translocation
Yoshihide Tsujimoto,Lawrence R. Finger,Jorge J. Yunis,Peter C. Nowell,Carlo M. Croce,Carlo M. Croce +5 more
TL;DR: In this paper, a DNA probe was obtained from an acute B-cell leukemia cell line, which was specific for chromosome 18 and flanked the heavy chain joining region of the immunoglobulin heavy chain locus on chromosome 14.
Journal ArticleDOI
The Chromosomal Basis of Human Neoplasia
TL;DR: It is proposed that chromosomal rearrangements play a central role in human neoplasia and may exert their effects through related genomic mechanisms and a translocation could serve to place an oncogene next to an activating DNA sequence, a deletion to eliminate anOncogene repressor, and trisomy to carry extra gene dosage.
Journal ArticleDOI
Structure of the human immunoglobulin mu locus: characterization of embryonic and rearranged J and D genes.
TL;DR: The variable portion of an immunoglobulin heavy chain gene is assembled from at least three discontinuous segments of DNA, the V, D and J regions, and the large number of human J region genes and, hence, their greater potential for generating diversity as compared to the that of the mouse J regions appears to result from recent genetic duplications.