Journal ArticleDOI
Structure of the human immunoglobulin mu locus: characterization of embryonic and rearranged J and D genes.
TLDR
The variable portion of an immunoglobulin heavy chain gene is assembled from at least three discontinuous segments of DNA, the V, D and J regions, and the large number of human J region genes and, hence, their greater potential for generating diversity as compared to the that of the mouse J regions appears to result from recent genetic duplications.About:
This article is published in Cell.The article was published on 1981-12-01. It has received 938 citations till now. The article focuses on the topics: Immunoglobulin heavy chain & Pseudogene.read more
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Canonical structures for the hypervariable regions of immunoglobulins
TL;DR: The relatively few residues that, through their packing, hydrogen bonding or the ability to assume unusual phi, psi or omega conformations, are primarily responsible for the main-chain conformations of the hypervariable regions are identified.
Journal ArticleDOI
Making Antibodies by Phage Display Technology
TL;DR: Human antibody fragments with many different binding specificities have been isolated from the same phage repertoire, including haptens, carbohydrates, secreted and cell surface proteins, viral coat proteins, and intracellular antigens from the lumen of the endoplasmic reticulum and the nucleus.
Journal ArticleDOI
Translocation of the c-myc gene into the immunoglobulin heavy chain locus in human Burkitt lymphoma and murine plasmacytoma cells
Rebecca Taub,Ilan R. Kirsch,Cynthia C. Morton,Gilbert M. Lenoir,D. Swan,Steven R. Tronick,Stuart A. Aaronson,Philip Leder +7 more
TL;DR: It is shown that transformation of human Burkitt lymphomas and murine plasmacytomas is frequently accompanied by the somatic rearrangement of a cellular analogue of an avian retrovirus transforming gene, c-myc, which provides a molecular basis for considering the role that specific translocations might play in malignant transformation.
Journal ArticleDOI
Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation
TL;DR: The results suggest that t(14;18) translocations alter expression of the bcl-2 gene both by transcriptional activation and by abnormal posttranscriptional regulation of bCl-2 mRNA.
Journal ArticleDOI
Cloning the chromosomal breakpoint of t(14;18) human lymphomas: clustering around Jh on chromosome 14 and near a transcriptional unit on 18
Ajay Bakhshi,Ajay Bakhshi,Jane P. Jensen,Jane P. Jensen,Paula L. Goldman,Paula L. Goldman,John J. Wright,John J. Wright,O. Wesley McBride,O. Wesley McBride,Alan L. Epstein,Alan L. Epstein,Stanley J. Korsmeyer,Stanley J. Korsmeyer +13 more
TL;DR: The t(14;18)(q32;q21) chromosomal translocation present in over 60% of human follicular lymphomas is characterized and an unexpected rearrangement of an Ig heavy-chain gene is exploited to clone the chromosomal breakpoint.
References
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DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
Book ChapterDOI
Sequencing end-labeled DNA with base-specific chemical cleavages.
Allan M. Maxam,Walter Gilbert +1 more
TL;DR: The chapter presents techniques for producing discrete DNA fragments, end-labeling DNA, segregating end- labeled fragments, extracting DNA from gels, and the protocols for partially cleaving it at specific bases using the chemical reactions.
Journal ArticleDOI
Screening lambdagt recombinant clones by hybridization to single plaques in situ
WD Benton,Ronald W. Davis +1 more
TL;DR: A rapid, direct method for screening single plaques of Agt recombinant phage is described, which allows at least 10(6) clones to be screened per day and simplifies physical containment of recombinants.
Journal ArticleDOI
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing
TL;DR: An approach to DNA sequencing using chain-terminating inhibitors (Sanger et al., 1977) combined with cloning of small fragments of DNA in a single-stranded DNA bacteriophage is described, determining the 2771-nucleotide sequence of the largest MboI restriction enzyme fragment from human mitochondrial DNA.
Journal ArticleDOI
The isolation of structural genes from libraries of eucaryotic DNA
Tom Maniatis,Ross C. Hardison,Elizabeth Lacy,Joyce Lauer,Catherine O'Connell,Diana Quon,Gek Kee Sim,Argiris Efstratiadis +7 more
TL;DR: A procedure for eucaryotic structural gene isolation which involves the construction and screening of cloned libraries of genomic DNA is presented and Restriction mapping and hybridization studies reveal the presence of closely linked beta-globin genes.