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Proceedings ArticleDOI

Compact silicon biosensor for the clinical range estimation of blood serum triglycéride

TL;DR: A miniaturized biochip with immobilized enzyme integrated with a thin film counter electrode for the estimation of TG in blood serum within the clinical range is developed.
Abstract: Electrolyte Insulator Semiconductor Capacitor (El S CAP) is a transducer that can detect triglycerides (TGs) in blood serum through an enzymatic response that changes the pH of the electrolyte (blood serum). This change in pH is transformed by the EISCAP to a shift in the capacitance-voltage (C-V) characteristics of the device which in turn can be mapped to the amount of TG in blood serum. We have developed a miniaturized biochip with immobilized enzyme integrated with a thin film counter electrode for the estimation of TG in blood serum within the clinical range. The miniaturized devices are tested using blood serum sample to estimate the TG concentration and compared with clinical data.
References
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Journal ArticleDOI
TL;DR: The development of an ion-sensitive solid-state device that combines the principles of an MOS transistor and a glass electrode and can be used for measurements of ion activities in electrochemical and biological environments is described.
Abstract: The development of an ion-sensitive solid-state device is described. The device combines the principles of an MOS transistor and a glass electrode and can be used for measurements of ion activities in electrochemical and biological environments. Some preliminary results are given.

1,841 citations


"Compact silicon biosensor for the c..." refers background in this paper

  • ...Use of solid state biosensors [1]-[5] ensures more accuracy and sensitivity compared to conventional sensors....

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Journal ArticleDOI
15 Nov 2006-Langmuir
TL;DR: A range of film morphologies based on the film deposition conditions are presented here to establish an optimized method of APTES film formation.
Abstract: Thin films of 3-aminopropyltriethoxysilane (APTES) are commonly used to promote adhesion between silica substrates and organic or metallic materials with applications ranging from advanced composites to biomolecular lab-on-a-chip. Unfortunately, there is confusion as to which reaction conditions will result in consistently aminated surfaces. A wide range of conflicting experimental methods are used with researchers often assuming the creation of smooth self-assembled monolayers. A range of film morphologies based on the film deposition conditions are presented here to establish an optimized method of APTES film formation. The effect of reaction temperature, solution concentration, and reaction time on the structure and morphology was studied for the system of APTES on silica. Three basic morphologies were observed: smooth thin film, smooth thick film, and roughened thick film.

668 citations

Journal ArticleDOI
TL;DR: A new solid-state device has been developed that makes it possible to measure ion activities without using a reference electrode and has the properties of both a glass electrode and a field-effect transistor.
Abstract: A new solid-state device has been developed for the measurement of ion activities in electrochemical and biological environments. One can recognize in the device the properties of both a glass electrode and a field-effect transistor. This justifies the name ion-sensitive field-effect transistor. The device makes it possible to measure ion activities without using a reference electrode. For its application, a special electronic circuit is described. Results of measured Na + and H + ion activities are given in detail. As an example for electrophysiological application, results are shown of recorded extracellular ion pulses measured with a guinea pig taenia coli.

588 citations

Journal ArticleDOI
TL;DR: In this article, an analysis of the quasi-equilibrium characteristics of an electrolyte-oxide-semiconductor (EOS) system has been carried out by initially considering two limiting cases: an ideal unblocked interface between the electrolyte and the oxide is assumed.
Abstract: In order to provide an improved understanding of ion-sensitive field-effect transistors (ISFET's) an analysis of the quasi-equilibrium characteristics of an electrolyte-oxide-semiconductor (EOS) system has been carried out. The characteristics of this system are examined by initially considering two limiting cases. In the first case, an ideal unblocked interface between the electrolyte and the oxide is assumed. Electrochemical processes of ion exchange dominates and consequently, a Nernstian response is experienced. The second limiting case assumes a totally blocked interface such that the behavior of the EOS system is dictated by electrostatic factors. The analysis is then generalized to examine the case of an electrolyte-pyrogenic SiO 2 -Si system using a site binding model to describe the ionic adsorption processes at the electrolyte-pyrogenic SiO 2 interface. The C-V characteristics and changes in flat-band voltage of the EOS system in response to pH are examined. Analytical results show that in general, the response does not obey the classical Nernst equation. Correlation of theoretical and experimental results yields excellent agreement suggesting that the models used in this study can provide an adequate description of the physical processes.

241 citations

Journal ArticleDOI
TL;DR: The hypothesis that LpL interacts with lipid interfaces to increase the catalytic activity toward a water-soluble substrate is supported, for interfacial activation, LnL prefers the lipid interface in the gel phase to that in the liquid-crystalline phase.

119 citations


"Compact silicon biosensor for the c..." refers methods in this paper

  • ...The free enzyme activity is determined by p-Nitro Phenol Butyrate (pNPB) assay [13] and used as a calibration data (Dcal) for the determination of immobilized enzyme activity....

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