Comparison of four molecular typing methods to assess genetic relatedness of Candida albicans clinical isolates in Taiwan.
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TLDR
The investigation of the genetic profiles of 53 Candida albicans isolates collected from 18 hospitals in Taiwan using three PFGE-based typing methods (PFGE karyotyping, and PFGE of SfiI and BssHII restriction fragments) and one repetitive-sequence-PCR (rep- PCR) method showed that the genotype of each isolate was patient-specific and not associated with the source of the isolation, geographic origin or antifungal resistance.Abstract:
This report describes the investigation of the genetic profiles of 53 Candida albicans isolates collected from 18 hospitals in Taiwan using three PFGE-based typing methods (PFGE karyotyping, and PFGE of SfiI and BssHII restriction fragments) and one repetitive-sequence-PCR (rep-PCR) method. All four methods were able to identify clonal related isolates from the same patients. PFGE-BssHII exhibited the highest discriminatory power by discriminating 40 genotypes, followed by PFGE-SfiI (35 genotypes) and then by rep-PCR (31 genotypes), while PFGE karyotyping exhibited the lowest discriminatory power (19 genotypes). High discriminatory power can also be achieved by combining typing methods with different typing mechanisms, such as rep-PCR and PFGE-based typing methods. The results also showed that the genotype of each isolate was patient-specific and not associated with the source of the isolation, geographic origin or antifungal resistance.read more
Citations
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Bacterial strain typing in the genomic era.
TL;DR: The applications of genotyping methods to the study of bacterial strain diversity are described and compared and the progresses allowed by the availability of genomic sequences are investigated.
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Candida colonisation as a source for candidaemia.
L.N. Miranda,I.M. van der Heijden,Silvia Figueiredo Costa,A.P.I. Sousa,R.A. Sienra,S. Gobara,C.R. Santos,Renata D. Lobo,V.P. Pessoa,Anna S. Levin +9 more
TL;DR: It is suggested that gastrointestinal colonisation is the probable source of C. albicans candidaemia and C. parapsilosis, both of which are found in patients with positive blood culture for candida.
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Global analysis of the evolution and mechanism of echinocandin resistance in Candida glabrata.
Sheena D. Singh-Babak,Tomas Babak,Stephanie Diezmann,Jessica A. Hill,Jinglin L. Xie,Ying-Lien Chen,Susan M. Poutanen,Susan M. Poutanen,Robert Rennie,Joseph Heitman,Leah E. Cowen +10 more
TL;DR: The first global analysis of mutations accompanying the evolution of fungal drug resistance in a human host utilizing a series of C. glabrata isolates is provided, implicate the premier compensatory mutation mitigating the cost of echinocandin resistance, and suggest a new mechanism of Echinoc andin resistance with broad therapeutic potential.
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Candida albicans Carriage in Children with Severe Early Childhood Caries (S-ECC) and Maternal Relatedness.
Jin Xiao,Yonghwi Moon,Lihua Li,Elena Rustchenko,Hironao Wakabayashi,Xiaoyi Zhao,Changyong Feng,Steven R. Gill,Sean W. McLaren,Hans Malmstrom,Yan-Fang Ren,Robert G. Quivey,Hyun Koo,Dorota T Kopycka-Kedzierawski +13 more
TL;DR: The results reveal that both the child with S-ECC and the mother were highly infected with C. albicans, while most of the strains were genetically related, suggesting that the mother might be a source for C.Albicans acquisition in the oral cavity of children affected by the disease.
Journal ArticleDOI
Multilocus Sequence Typing for Analyses of Clonality of Candida albicans Strains in Taiwan
TL;DR: The result showed that the DNA type of each isolate was patient specific and associated with ABC type and decade of isolation but not associated with mating type, anatomical source of isolation, hospital origin, or fluconazole resistance patterns.
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