Journal ArticleDOI
Comparison of the RE and B1 gene for detection of Toxoplasma gondii infection in children with cancer
Sh. Fallahi,Bahram Kazemi,Seyyed Javad Seyyed Tabaei,Mojgan Bandehpour,Zohreh Lasjerdi,Niloofar Taghipour,Nozhat Zebardast,Bahram Nikmanesh,V. Fallah Omrani,Farzad Ebrahimzadeh +9 more
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TLDR
The results indicate that an RE-based nested PCR assay is more sensitive than B1 genomic target, of those tested, for detection of T. gondii.About:
This article is published in Parasitology International.The article was published on 2014-02-01. It has received 59 citations till now. The article focuses on the topics: Polymerase chain reaction & Toxoplasma gondii.read more
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Diagnosis of toxoplasmosis and typing of Toxoplasma gondii
TL;DR: Conventional non-DNA-based diagnostic methods and DNA-based molecular techniques have provided foundations for further development of more effective and accurate detection of T. gondii infection and will contribute to an improved understanding of the epidemiology, prevention and control of toxoplasmosis.
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Comparison of loop-mediated isothermal amplification (LAMP) and nested-PCR assay targeting the RE and B1 gene for detection of Toxoplasma gondii in blood samples of children with leukaemia.
Shirzad Fallahi,Shirzad Fallahi,Seyyed Javad Seyyed Tabaei,Yadollah Pournia,Nozhat Zebardast,Bahram Kazemi +5 more
TL;DR: This is the first report of a study in which the LAMP method was applied with high sensitivity and efficacy for the diagnosis of T. gonii in blood samples of children with leukaemia.
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Challenging loop—mediated isothermal amplification (LAMP) technique for molecular detection of Toxoplasma gondii
TL;DR: The obtained results demonstrate that the LAMP technique has a greater sensitivity for detection of T. gondii DNA and indicate that primers based on the RE are more suitable than thosebased on the B1 gene.
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Immunodiagnosis and molecular validation of Toxoplasma gondii-recombinant dense granular (GRA) 7 protein for the detection of toxoplasmosis in patients with cancer.
TL;DR: Findings of the present study showed that rGRA7 can be used as a potential immunogenic antigen for developing immunodiagnostic tools for Immunodiagnosis of toxoplasmosis in patients including patients with cancer.
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Prevalence and genotyping of Toxoplasma gondii among Saudi pregnant women in Saudi Arabia
TL;DR: Defining the population structure of T. gondii from Saudi Arabia has important implications for transmission, immunogenicity, pathogenesis, and in planning preventive strategies and might facilitate and inform future studies on comparative genomics and identification of genes that control important biological phenotypes.
References
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Journal ArticleDOI
Direct and sensitive detection of a pathogenic protozoan, Toxoplasma gondii, by polymerase chain reaction.
TL;DR: A combination of sensitivity and specificity should make detection of the B1 gene based on polymerase chain reaction amplification a very useful method for diagnosis of toxoplasmosis both in immunocompromised hosts and in congenitally infected fetuses.
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Toxoplasmosis - a waterborne zoonosis.
TL;DR: Information on the biology of oocyst-induced infections of T. gondii in humans and animals is reviewed and possible importance of transmission by water is examined.
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Identification of a 200- to 300-fold repetitive 529 bp DNA fragment in Toxoplasma gondii, and its use for diagnostic and quantitative PCR.
TL;DR: Quantitative competitive-PCR with the 529bp fragment can be used as an alternative for the tedious visual counting of brain cysts in experimental animals and could confirm the copy number of the 529BP fragment in tachyzoites and estimate the number of bradyzoites per cyst.
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Polymerase chain reaction.
Lilit Garibyan,Nidhi Avashia +1 more
TL;DR: It is important to understand the basic principles of PCR and how its use can be modified to provide for sophisticated analysis of genes and the genome.