Journal ArticleDOI
Comparison of two liposome fusion assays monitoring the intermixing of aqueous contents and of membrane components
TLDR
Mg2+ inhibited the Ca2+-induced fusion of PS vesicles, but did not cause any fusion by itself, consistent with previous results with the Tb/dipicolinic acid assay.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1983-10-26. It has received 54 citations till now. The article focuses on the topics: Lipid bilayer fusion & Vesicle.read more
Citations
More filters
Journal ArticleDOI
H+- and Ca2+-induced fusion and destabilization of liposomes
TL;DR: It is shown that H+ causes lipid mixing in this pH range but not mixing of aqueous contents, which affirms the necessity of using both aqueously space and lipid bilayer assays to comprehend the fusion event between two liposomes.
Journal ArticleDOI
Lipids in biological membrane fusion.
TL;DR: The results reviewed suggest that membrane fusion in diverse biological fusion reactions involves formation of some specific intermediates: stalks and pores, and suggest a specific geometry to bent fusion intermediates (stalks and pores) and imply a contribution by lipids to the energy of these intermediates.
Journal ArticleDOI
Phosphatidylethanolamine liposomes: drug delivery, gene transfer and immunodiagnostic applications.
David C. Litzinger,Leaf Huang +1 more
TL;DR: The physical proper and physical proper behavior of PE and PE l i r~somes are studied, as well as the Divalent cation effect, which describes theivalent effect of pH in PE systems.
Journal ArticleDOI
Effects of a surfactant-associated protein and calcium ions on the structure and surface activity of lung surfactant lipids.
TL;DR: Results add to the growing evidence that specific Ca2+-protein-lipid interactions are important in determining both the structure and function of extracellular lung surfactant fractions.
Journal ArticleDOI
Molecular mechanisms of calcium-induced membrane fusion.
TL;DR: A simple molecular model is presented for the mechanism of lipid membrane fusion, consistent with the experimental evidence and incorporating various elements proposed previously.
References
More filters
Journal ArticleDOI
Use of resonance energy-transfer to monitor membrane-fusion
TL;DR: An assay for vesicle--vesicle fusion involving resonance energy transfer between N-(7-nitro-2,1,3-benzoxadiazol-4-yl), the energy donor, and rhodamine, the energy acceptor has been developed.
Journal ArticleDOI
Cochleate lipid cylinders: formation by fusion of unilamellar lipid vesicles.
TL;DR: The name cochleate lipid cylinders is suggested for the spiral structures formed by fusion of unilamellar vesicles into large sheets which fold spirally to form cylinders in phosphatidylserine preparations.
Journal ArticleDOI
Studies on the mechanism of membrane fusion: kinetics of calcium ion induced fusion of phosphatidylserine vesicles followed by a new assay for mixing of aqueous vesicle contents.
TL;DR: This work has studied the Ca2+-induced fusion of small or large unilamellar vesicles (SUV or LUV, respectively) composed of phosphatidylserine (PS), and found that at high vesicle concentrations (and at relatively low Ca2- concentrations) aggregation may proceed faster than fusion.
Journal ArticleDOI
Adsorption of divalent cations to bilayer membranes containing phosphatidylserine
TL;DR: The Stern equation, a combination of the Langmuir adsorption isotherm, the Boltzmann relation, and the Grahame equation from the theory of the diffuse double layer, provides a simple theoretical framework for describing the adsor adaptation of charged molecules to surfaces.
Journal ArticleDOI
Studies on membrane fusion. II. Induction of fusion in pure phospholipid membranes by calcium ions and other divalent metals
TL;DR: It is suggested that under these conditions membranes become transiently susceptible to fusion as a result of changes in molecular packing and creation of new phase boundaries induced by Ca2+ (or Mg2+).