Journal ArticleDOI
Complementary RNAs in paramyxovirions and paramyxovirus-infected cells.
Allen Portner,David W. Kingsbury +1 more
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Paramyxoviruses contain single-stranded RNAs sedimenting at 50–57S1–3, Newcastle disease virus (NDV) and Sendai virus have been shown to induce infected cells to synthesize single-strate RNAs which sediment more slowly than virion RNAs and are complementary to them in nucleotide sequences4–6.Abstract:
PARAMYXOVIRIONS contain single-stranded RNAs sedimenting at 50–57S1–3, Newcastle disease virus (NDV) and Sendai virus have been shown to induce infected cells to synthesize single-stranded RNAs which sediment more slowly than virion RNAs and are complementary to them in nucleotide sequences4–6. Interest in these complementary RNAs derives from the possibility that they are templates for viral proteins. For some RNA viruses it is clear that the viral genome is the message7–10; but for paramyxoviruses, the genome may contain little or no genetic information that can be translated directly. Paramyxoviruses do not seem to be unique: vesicular stomatitis virus (VSV) directs the synthesis of RNAs smaller than virion RNA and complementary to it11,12.read more
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Book ChapterDOI
Reproduction of Paramyxoviruses
TL;DR: Measles, canine distemper, and rinderpest viruses form a distinct subgroup on the basis of antigenicity, hemagglutinating characteristics, and lack of evidence for a virion-associated neuraminidase or neuraminic acid-containing cellular receptors, but it is now generally accepted that these viruses should be included in the paramyxovirus group because of their similar structural properties.
Book ChapterDOI
Defective Interfering Animal Viruses
Alice S. Huang,David Baltimore +1 more
TL;DR: Defective interfering particles were discovered three decades ago by von Magnus using the influenza virus system and a great deal was learned from their physiological interactions with the host and from their interference with the multiplication of “infectious” standard virus.
Journal ArticleDOI
RNA of RNA Tumour Viruses contains Poly A
TL;DR: Molecular hybridization with radioactive polyuridylic acid has been used to detect regions of polyadenylic acid in virus RNA to make a qualitative distinction between RNA preparations from the two virus classes.
Journal ArticleDOI
Ribonucleic Acud Polymerase in Virions of Newcastle Disease Virus: Comparison with the Vesicular Stomatitis Virus Polymerase
Alice S. Huang,Alice S. Huang,David Baltimore,David Baltimore,Michael A. Bratt,Michael A. Bratt +5 more
TL;DR: The virions of Newcastle disease virus (NDV) contained an enzyme that catalyzed the incorporation of ribonucleotides into RNA, and the optimal conditions for this enzyme were identical to the conditions for the vesicular stomatitis virus (VSV) polymerase, and both enzymes were active for longer times at 32 C than at 37 C as discussed by the authors.
Journal ArticleDOI
Further characterization of Sendai virus DI-RNAs: a model for their generation.
TL;DR: A genetic map and a model for the generation of the Sendai DI-RNAs are presented, showing that almost all the sequences contained in the DI- RNAs derive from sequences that are contiguous to the 5' end of the nondefective (ND) genome.
References
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Journal ArticleDOI
A cytoplasmic structure involved in the synthesis and assembly of poliovirus components.
TL;DR: A cytoplasmic structure involved in the synthesis and maturation of poliovirus has been demonstrated in infected HeLa cells and a lag time of at least 20 minutes for amino acids and 10 minutes for uridine precedes their appearance in mature virus.
Journal ArticleDOI
Ribonucleic acid synthesis in cells infected with Newcastle disease virus
TL;DR: All four labeled RNA components are found almost exclusively in the cytoplasm after incubating infected cells with [3H]uridine in the presence of actinomycin D for 15 minutes and most of the viral specific RNA is attached to polyribosomes in the cell.
Journal ArticleDOI
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Journal ArticleDOI
Replication of viral ribonucleic acid: IX. Properties of double-stranded RNA from Escherichia coli infected with bacteriophage MS2
TL;DR: The double-stranded, MS2-specifie RNA formed in Escherichia coli after infection with bacteriophage MS2 has been purified on a large scale by a method involving extensive treatment with ribonuclease.