Complex dynamics of multicomponent biological coacervates
Jenna K.A. Tom,Ashok A. Deniz +1 more
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TLDR
The fundamental concepts discussed in this review are widely applicable, including in the context of cellular function and development of materials with novel properties in the areas of dynamics and multicomponent systems.Abstract:
Phase transitions and coacervates play key roles in natural and synthetic soft matter. In particular, the past few years have seen a rapid expansion in studies of these phenomena in the context of dynamic cellular compartmentalization. In this brief review, we mainly focus on a few concepts and selected in vitro and cellular examples of recent developments in the areas of dynamics and multicomponent systems. Topics covered include the flexibility and conformational dynamics of polymeric species involved in phase separation, valence and non-monotonic effects, noise modulation and feedback loops, and multicomponent systems and substructure. The fundamental concepts discussed in this review are widely applicable, including in the context of cellular function and the development of materials with novel properties.read more
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Liquid-liquid phase separation of α-Synuclein: A new mechanistic insight for α-Synuclein aggregation associated with Parkinson's disease pathogenesis.
Semanti Mukherjee,A. Sakunthala,Laxmikant G. Gadhe,Manisha Poudyal,Ajay Singh Sawner,Pradeep Kadu,Samir K. Maji +6 more
TL;DR: In this article , the authors demonstrate the recent development of α-Syn LLPS, the underlying mechanism along with the microscopic events of aberrant phase transition, and further discuss how several intrinsic and extrinsic factors regulate the thermodynamics and kinetics of αSynLLPS and co-LLPS with other proteins, which might explain the pathophysiology of α Syn in various neurodegenerative diseases.
Journal ArticleDOI
Engineering coacervate droplets towards the building of multiplex biomimetic protocells
Hua Sheng Wu,Yan Qiao +1 more
TL;DR: A recent tutorial review as mentioned in this paper summarizes the recent progress on engineering coacervate protocells to reproduce the function and structure of primitive life by multiphase organization, membranization and structural hybridization.
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Percolation physics and density transition frameworks converge in biomolecular condensation
TL;DR: Kar et al. as mentioned in this paper proposed a percolation-based model of biomolecular condensates and showed that protein clusters follow a heavytailed distribution, with low abundance of larger mesoscale clusters and distributions changing with total protein concentration.
Journal ArticleDOI
Conformational Freedom and Topological Confinement of Proteins in Biomolecular Condensates.
Daniel Scholl,Ashok A. Deniz +1 more
TL;DR: In this article, the authors summarize and discuss the findings of several recent studies that have focused on structure, dynamics, and interactions of proteins undergoing condensation and speculate on effects of topological constraints and physical exclusion on condensate properties.
Journal ArticleDOI
Conformational Freedom and Topological Confinement of Proteins in Biomolecular Condensates
TL;DR: In this article , the authors summarize and discuss the findings of several recent studies that have focused on structure, dynamics, and interactions of proteins undergoing condensation and speculate on effects of topological constraints and physical exclusion on condensate properties.
References
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Journal ArticleDOI
Role of Chain Flexibility in Asymmetric Polyelectrolyte Complexation in Salt Solutions
TL;DR: In this paper, the role of DNA local flexibility in governing liquid-liquid phase separation (LLPS) is investigated, and the results show that when both the polyanion and polycation are flexible (flexible-flexible complex), denser complexes with a higher degree of structural correlation form, with fewer free chains released into the supernatant, compared to the case when the poly anion is rigid (rigid-flexibility complex), in agreement with the LLPS experiments.
Posted ContentDOI
Decoding the physical principles of two-component biomolecular phase separation
TL;DR: An analytical dimer-gel theory was developed that confirmed the magic-ratio effect and disentangled the individual roles of polymer properties in shaping the phase diagram and provides new insights into the factors controlling the phase diagrams of biomolecular condensates, with implications for natural and synthetic systems.
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Divalent cations can control a switch-like behavior in heterotypic and homotypic RNA coacervates
TL;DR: It is predicted and observed that variations in signaling cations exert interaction-dependent effects on RNA LLPS, and the results may provide a general mechanism for modulating the biochemical environment of RNA coacervates in a cellular context.
Journal ArticleDOI
Magic numbers in polymer phase separation -- the importance of being rigid
Bin Xu,Guanhua He,Benjamin G. Weiner,Pierre Ronceray,Yigal Meir,Martin C. Jonikas,Ned S. Wingreen +6 more
TL;DR: In this article, the magic-number effect was shown to be enhanced if one of the polymer species has a rigid shape that allows for multiple distinct bonding conformations, and if one species is rigid, the effect is robust over a much greater range of relative concentrations of the two species.
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On the resilience of magic number theory for conductance ratios of aromatic molecules.
Lara Ulčakar,T. Rejec,T. Rejec,Jure Kokalj,Jure Kokalj,Sara Sangtarash,Hatef Sadeghi,Anton Ramšak,Anton Ramšak,John H. Jefferson,Colin J. Lambert +10 more
Abstract: If simple guidelines could be established for understanding how quantum interference (QI) can be exploited to control the flow of electricity through single molecules, then new functional molecules, which exploit room-temperature QI could be rapidly identified and subsequently screened. Recently it was demonstrated that conductance ratios of molecules with aromatic cores, with different connectivities to electrodes, can be predicted using a simple and easy-to-use “magic number theory.” In contrast with counting rules and “curly-arrow” descriptions of destructive QI, magic number theory captures the many forms of constructive QI, which can occur in molecular cores. Here we address the question of how conductance ratios are affected by electron-electron interactions. We find that due to cancellations of opposing trends, when Coulomb interactions and screening due to electrodes are switched on, conductance ratios are rather resilient. Consequently, qualitative trends in conductance ratios of molecules with extended pi systems can be predicted using simple ‘non-interacting’ magic number tables, without the need for large-scale computations. On the other hand, for certain connectivities, deviations from non-interacting conductance ratios can be significant and therefore such connectivities are of interest for probing the interplay between Coulomb interactions, connectivity and QI in single-molecule electron transport.