Journal ArticleDOI
Control mechanisms for gonadotrophic hormone production in vitro
R. A. Pattillo,R. A. Pattillo,R. O. Hussa,R. O. Hussa,Eleanor Delfs,Eleanor Delfs,J. Garancis,J. Garancis,R. Bernstein,R. Bernstein,A. C. F. Ruckert,A. C. F. Ruckert,W. Y. Huang,W. Y. Huang,George O. Gey,George O. Gey,Richard F. Mattingly,Richard F. Mattingly +17 more
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TLDR
Biological, biochemical, and electron microscopic findings verify inhibition of gonadotrophic hormone synthesis, depletion of glycogen, activation of phosphorylase, the glycogen-metabolizing enzyme, and ultrastructural appearance of liquid-containing cytoplasmic granules in single cells, indicative of steroid synthesis.Abstract:
An internal control mechanism capable of controlling gonadotrophic hormone secretion in the human trophoblast has been identified by incubation of an established cell line with a precursor of progesterone. Biological, biochemical, and electron microscopic findings verify inhibition of gonadotrophic hormone synthesis, depletion of glycogen, activation of phosphorylase, the glycogen-metabolizing enzyme, and ultrastructural appearance of liquid-containing cytoplasmic granules in single cells, indicative of steroid synthesis.read more
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Relationship of oral contraception to development of trophoblastic tumour after evacuation of a hydatidiform mole
TL;DR: The need for chemotherapy for trophoblastic tumour after evacuation of a hydatidiform mole was found to be significantly increased in patients taking oral contraceptives before normal human chorionic gonadotrophin (HCG) values were obtained.
Journal ArticleDOI
Tissue-specific expression and thyroid hormone regulation of the endogenous placental growth hormone variant and chorionic somatomammotropin genes in a human choriocarcinoma cell line.
TL;DR: BeWo cells provide a model system for studying hGH-V and hCS regulation as well as tissue-specific expression and treatment of BeWo cells with 10 nM T3 results in a 6-fold increase in messenger RNA from placental members of the hGH gene family.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal Article
The Establishment of a Cell Line of Human Hormone-synthesizing Trophoblastic Cells in Vitro
Roland A. Pattillo,George O. Gey +1 more
TL;DR: A human hormone-synthesizing trophoblastic cell system has been established in vitro and may prove to be the first functional human embryonic cell line in continuous culture and is being stabilized in serial cultivation.
Journal ArticleDOI
Isolation of hormone-producing clonal lines of human choriocarcinoma
TL;DR: Six clonal lines of human choriocarcinoma have been established from a tumor previously adapted to passage in the hamster cheek pouch, providing systems for the examination of many aspects of placental physiology and biology.
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The Establishment of a Cell Line of Human Hormone-synthesizing Trophoblastic Cells in Vitro
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