Journal ArticleDOI
Core substructure in cyanobacterial phycobilisomes
TLDR
It is proposed that the two cylinders of the Synechocystis 6701 core, proximal to the thylakoid membrane, each have the composition ABCD, and that the distal cylinder has the composition A2B2.Abstract:
The tricylindrical core of Synechocystis 6701 phycobilisomes is made up of four types of allophycocyanin-containing complexes: A, (αAP βAP)3; B, (αAP βAP)3 .10K; C, (ααβ).10K; D, (αAP βAP)2.18.5K.99K; where AP is allophycocyanin, APB is allophycocyanin B, and 10K, 18.5K, and 99K are polypeptides of 10,000, 18,500, and 99,000 daltons, respectively. The 18.5K polypeptide is a hitherto unrecognized biliprotein subunit with a single phycocyanobilin prosthetic group. The tricylindrical core is made up of 12 subcomplexes in the molar ratio of A:B:C:D: of 4:4:2:2. Complexes C and D act as terminal energy acceptors. From these results and previous analysis of the bicylindrical core of Synechococcus 6301 phycobilisomes [14,15] it is proposed that the two cylinders of the Synechocystis 6701 core, proximal to the thylakoid membrane, each have the composition ABCD, and that the distal cylinder has the composition A2B2.read more
Citations
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Book ChapterDOI
Phycobilisome and Phycobiliprotein Structures
TL;DR: Phycobilisomes serve as the primary light-harvesting antennae for Photosystem II in cyanobacteria and red algae and contain smaller amounts ‘linker polypeptides,’ most of which do not bear chromophores.
Journal ArticleDOI
Structure and function of light‐harvesting complexes and their polypeptides
TL;DR: In this article, a detailed biochemical and structural characterization of the pigment protein complexes of light harvesting antennae was obtained. And the detailed biochemical analysis also allowed a better identification of the various pigmentprotein complexes, which is essential for their isolation.
Journal ArticleDOI
The "anchor polypeptide" of cyanobacterial phycobilisomes. Molecular characterization of the Synechococcus sp. PCC 6301 apce gene.
TL;DR: The functional organization of the apcEABC gene cluster, as well as of theApcE gene product, of Synechococcus 6301 are compared to their counterparts in three other organisms and a model is proposed for the architecture of the phycobilisome core.
Journal ArticleDOI
Allophycocyanin and energy transfer
TL;DR: Although evidence supporting both models has been produced, sophisticated ultra fast fluorescence results from a plethora of approaches now firmly point to the latter strong coupling hypothesis as being more likely.
References
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Journal ArticleDOI
Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
TL;DR: A rapid and convenient method for peptide mapping of proteins has been developed that involves partial enzymatic proteolysis in the presence of sodium dodecyl sulfate and analysis of the cleavage products by polyacrylamide gel electrophoresis.
Journal ArticleDOI
The structure of cyanobacterial phycobilisomes: a model
Donald A. Bryant,Gerard Guglielmi,Nicole Tandeau de Marsac,Anne Marie Castets,Germaine Cohen-Bazire +4 more
TL;DR: In the present study, the hemi-discoidal phycobilisomes of several species of cyanobacteria were examined in thin sections of cells and by negative staining after isolation and fixation and it was suggested that the triangular core was composed of allophycocyanin and that the peripheral rods contained phyCocyan in and phycoerythrin (when present).
Book ChapterDOI
Structure and Function of Phycobilisomes: Light Harvesting Pigment Complexes in Red and Blue-Green Algae
TL;DR: This chapter focuses on the phycobilisome characteristics, their relationship to the photosystems in the thylakoid membrane, and major problems to be addressed in future investigations.
Journal ArticleDOI
Dynamic aspects of phycobilisome structure
TL;DR: In exponentially growing cells of Synechococcus sp.