Correlation between human sperm swelling in hypoosmotic medium (hypoosmotic swelling test) and in vitro fertilization.
H. van der Ven,R. S. Jeyendran,Safaa Al-Hasani,M. Perez-Pelaez,Klaus Diedrich,Lourens J.D. Zaneveld +5 more
TLDR
It appears that the hypoosmotic swelling test is a more accurate predictor of successful in vitro fertilization outcome than the conventional semen parameters.Abstract:
Human ejaculates (n = 83) were analyzed for standard sperm parameters (concentration, motility, and morphology), as well as for the ability of the spermatozoa to react (swell) in a hypoosmotic medium (Jeyendran et al, 1984) Subsequently, the fertilizing capacity of the spermatozoa was tested by their ability to fertilize human oocytes in vitro Although the sperm concentration was adjusted for in vitro fertilization, no adjustments were made for sperm motility and morphology Correlation of the in vitro fertilizing capacity of the spermatozoa with the hypoosmotic swelling test (r = 056) was much higher than with standard sperm parameters (r varied from -004 to 025) Complete overlap was noted with standard semen parameters whether the ejaculate did or did not fertilize oocytes and ranged from very low to very high values in both cases By contrast, all the semen samples that fertilized oocytes showed a 60% or higher reaction in the hypoosmotic swelling test, whereas the majority of the "infertile" semen samples showed less than 60% swelling It therefore appears that, under the conditions of our studies, the hypoosmotic swelling test is a more accurate predictor of successful in vitro fertilization outcome than the conventional semen parameters A combination of all parameters, however, is likely to be most useful The hypoosmotic swelling test is simple and economical, and it is recommended that this test be further scrutinized for its value as an additional tool in the assessment of the in vivo fertilizing capacity of ejaculated spermatozoaread more
Citations
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An osmotic resistance test for bovine semen
S.G. Revell,Raphael Mrode +1 more
TL;DR: The osmotic resistance test (ORT) was developed for bull semen and shows promise as a routine assessment technique in commercial AI laboratories as the method is simple and rapid.
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Bull semen in vitro fertility after cryopreservation using egg yolk LDL: a comparison with Optidyl®, a commercial egg yolk extender
Lamia Amirat,Daniel Tainturier,Laëtitia Jeanneau,Chantal Thorin,Olivier Gérard,J. L. Courtens,Marc Anton +6 more
TL;DR: Low-density lipoproteins preserve bull semen quality and fertilizing ability, allowing also better semen motility, after the freeze-thaw process, in conclusion.
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Leukocytospermia is associated with poor semen quality
Hans Wolff,Joseph A. Politch,Adriana Martinez,Florina Haimovici,Joseph A. Hill,Deborah J. Anderson +5 more
TL;DR: In this article, sperm samples from 179 male infertility patients were analyzed for type and number of white blood cells (WBC) by a combination of immunologic techniques and showed that elevated levels of WBC in semen are associated with poor semen quality.
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The hypoosmotic swelling test: Its employment as an assay to evaluate the functional integrity of the frozen-thawed bovine sperm membrane
J.R. Correa,Panayiotis M. Zavos +1 more
TL;DR: The results obtained in this study indicate that frozen-thawed bovine spermatozoa did react to the HOS test, and this technique could prove useful in studies involving the function of the sperm membrane and could possibly predict the sperm's ability to fertilize.
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The predictive value of semen analysis in the evaluation of stallion fertility.
TL;DR: Flow-cytometric analysis of sperm with appropriate probes offers considerable promise for the prediction of stallion fertility because it allows the objective, rapid and simultaneous analysis of a number of properties in a large number of sperm.
References
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Laboratory manual for the examination of human semen and semen-cervical mucus interaction.
TL;DR: This laboratory manual consists of 2 sections which describe methods of examination of human semen and semen-cervical mucus interaction in order to standardize procedures and facilitate evaluation and comparison of research reports.
Journal ArticleDOI
Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics
TL;DR: The hypoosmotic swelling technique to evaluate the functional integrity of the sperm membrane appears to give high repeatability and accuracy and is closely correlated to the in-vitro fertilizing ability of spermatozoa.
Journal ArticleDOI
Pregnancy following intrauterine implantation of an embryo obtained by in vitro fertilization of a preovulatory egg.
Alexander Lopata,Alexander Lopata,Ian W.H. Johnston,Ian W.H. Johnston,Ian J. Hoult,Ian J. Hoult,Andrew I. Speirs,Andrew I. Speirs +7 more
TL;DR: Elevated levels of serum human chorionic gonadotropin and progressively increasing levels of total urinary estrogens and pregnanediol confirmed the presence of an early pregnancy.
Journal ArticleDOI
Treatment-Independent Pregnancy among Infertile Couples
TL;DR: It is concluded that the potential for a spontaneous cure of infertility is high, that treatment for many classes of infertility should be evaluated by randomized clinical trials, and that in such trials random assignment of subjects to untreated control groups would be ethically acceptable.
Journal ArticleDOI
What functions of the sperm cell are measured by in vitro fertilization of zona-free hamster eggs?
John E. Gould,John E. Gould,James W. Overstreet,James W. Overstreet,H. Yanagimachi,H. Yanagimachi,Ryuzo Yanagimachi,Ryuzo Yanagimachi,David F. Katz,David F. Katz,Frederick W. Hanson,Frederick W. Hanson +11 more
TL;DR: In this paper, human spermatozoa were capacitated in media containing either high concentrations (3.5%) of human serum albumin (HSA) or low concentrations (0.3%) of Bovine Serrum Albumin (BSA), and the effects of both capacitation media were assessed immediately and after overnight preincubation by adding a mixture of nonliving human oocytes and living zona-free hamster eggs to the sperm suspension.