Journal ArticleDOI
Cryopreservation of human spermatozoa. III. The effect of cryoprotectants on motility.
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The data suggest that avoiding glycerol toxicity either by reducing the concentration used or by adding Glycerol at a lower temperature, or both, may improve human sperm cryosurvival rates.About:
This article is published in Fertility and Sterility.The article was published on 1988-08-01. It has received 131 citations till now. The article focuses on the topics: Sperm motility & Cryoprotectant.read more
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Role of antioxidants in treatment of male infertility: an overview of the literature
TL;DR: It was found that although many clinical trials have demonstrated the beneficial effects of antioxidants in selected cases of male infertility, some studies failed to demonstrate the same benefit, and conclusive evidence on the benefit of antioxidants as a treatment modality for patients with male infertility is still needed.
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Mechanisms of cryoinjury in living cells.
Doyong Gao,J. K. Critser +1 more
TL;DR: Factors and mechanisms of cryoinjury and its prevention are reviewed and discussed, including the most famous two-factor hypothesis theory of Peter Mazur, concepts of cold shock, vitrification, cryoprotective agens, lethal intracellular ice formation, osmotic injury during the addition/removal of CPAs and during the cooling/warming process, as well as modeling/methods in the cryobiological research.
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Recent progress in the preservation of ram semen
W.M.C. Maxwell,P.F. Watson +1 more
TL;DR: Stored spermatozoa may require less capacitation time in the female tract, and may readily fertilise oocytes if placed in their immediate vicinity, as with in vitro fertilisation, tubal or even intrauterine insemination.
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Potential Importance of Vitrification in Reproductive Medicine
Juergen Liebermann,Frank Nawroth,Vladimir Isachenko,Evgenia Isachenko,Gohar Rahimi,Michael J. Tucker +5 more
TL;DR: Vitrification as a cryopreservation method has had very little practical impact on human-assisted reproduction, and human preimplantation embryo vitrification is still considered to be largely experimental.
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Assessment of DNA integrity and morphology of ejaculated spermatozoa from fertile and infertile men before and after cryopreservation
TL;DR: Investigation of the effects of cryopreservation on both DNA integrity and morphology of spermatozoa from fertile and infertile men found it had a detrimental effect on morphology of semen and prepared samples from fertile, and sperm morphology was significantly damaged by freeze-thawing.
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The relevance of cryoprotectant "toxicity" to cryobiology.
TL;DR: It is suggested that better suppression of toxicity is possible and will lead to advances in cryopreservation, and the toxicity of these agents is a key limiting factor in cryobiology.
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Differing actions of penetrating and nonpenetrating cryoprotective agents
TL;DR: A two-step freezing technique has been used to examine the role of cryoprotective agents during cooling in Chinese hamster fibroblasts and indicates that penetrating and nonpenetrating agents accomplish this in different ways.
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Effect of Cryoprotective Media and Dilution Methods on the Preservation of Human Spermatozoa
TL;DR: Der Einfluß von kryprotektiven Medien und Verdünnungsmedien auf die Erhaltung menschlicher Spermatozoen ist bekannt.