Journal ArticleDOI
Cryptic speciation in Pelobates fuscus (Anura, Pelobatidae): evidence from DNA flow cytometry
Rosanov,Borkin,Milto,Litvinchuk +3 more
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The amount of nuclear DNA in 173 specimens of Pelobates fuscus from 34 localities in Russia, Ukraine, Moldavia and Latvia was determined by DNA cytometry, and two distinct groups with different genome sizes were identified.Abstract:
The amount of nuclear DNA in 173 specimens of Pelobates f. fuscus from 34 localities in Russia, Ukraine, Moldavia and Latvia was determined by DNA e ow cytometry. Two distinct groups with different genome sizes were identie ed. The ranges of the genome size variation in the two groups did not overlap. Geographically, these groups with smaller or larger genome size are distributed in the west and in the east of eastern Europe, respectively.read more
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Applications of Flow Cytometry to Evolutionary and Population Biology
TL;DR: The nature ofFlow cytometry contributions, as well as future applications, are outlined, and an online summary of protocols and sampling methods are provided.
Journal ArticleDOI
Species list of the European herpetofauna – 2020 update by the Taxonomic Committee of the Societas Europaea Herpetologica
Jeroen Speybroeck,Wouter Beukema,Christophe Dufresnes,Uwe Fritz,Daniel Jablonski,Petros Lymberakis,Iñigo Martínez-Solano,Edoardo Razzetti,Melita Vamberger,Miguel Vences,Judit Vörös,Pierre-André Crochet +11 more
TL;DR: According to the list, 301 species (95 amphibians, 15 chelonians, including six species of sea turtles, and 191 squamates) occur within the expanded geographical definition of Europe, which includes 14 non-native species.
Journal ArticleDOI
Fifteen shades of green: The evolution of Bufotes toads revisited.
Christophe Dufresnes,Glib Mazepa,Daniel Jablonski,Ricardo Caliari Oliveira,Tom Wenseleers,D. A. Shabanov,Markus Auer,Raffael Ernst,Claudia Koch,Héctor E. Ramírez-Chaves,Héctor E. Ramírez-Chaves,Kevin P. Mulder,Kevin P. Mulder,Kevin P. Mulder,Evgeniy Simonov,Arthur Tiutenko,Dmytro Kryvokhyzha,Paul Louis Wennekes,Oleksandr Zinenko,Oleksiy V. Korshunov,Awadh M. Al-Johany,Evgeniy A. Peregontsev,Rafaqat Masroor,Caroline Betto-Colliard,Mathieu Denoël,Leo J. Borkin,Dmitriy V. Skorinov,Roza A. Pasynkova,Lyudmila F. Mazanaeva,Juriy Rosanov,Sylvain Dubey,Spartak N. Litvinchuk +31 more
TL;DR: Across the radiation of Palearctic green toads, a stepwise progression of reproductive isolation through time is pinpointed, with a threshold below which hybridizability is irrespective of divergence, above which species barely admix and eventually evolve different mating calls, or can successfully cross-breed through allopolyploidization.
Journal ArticleDOI
Fossorial but widespread: the phylogeography of the common spadefoot toad (Pelobates fuscus), and the role of the Po Valley as a major source of genetic variability
Angelica Crottini,Franco Andreone,Joachim Kosuch,Joachim Kosuch,Leo J. Borkin,Spartak N. Litvinchuk,Christophe Eggert,Michael Veith,Michael Veith +8 more
TL;DR: This work sequenced 702 bp of the mitochondrial cytochrome b gene and estimated the divergence of these phylogeographical lineages to the Pliocene to fit the hypothesis that climatic fluctuation was a key event for differentiation processes in P. fuscus.
Journal ArticleDOI
Historical biogeography of Western Palaearctic pelobatid and pelodytid frogs: a molecular phylogenetic perspective
Abstract: Spadefoot toads (Pelobates) and Parsley frogs (Pelodytes) are an enigmatic group of Western Palaearctic anurans. In the genus Pelobates, a fossorial lifestyle has enforced a conserved bauplan that masks their intraspecifi c evolutionary history. We used partial sequences of the mitochondrial 16S and 12S rRNA genes to infer a paleobiogeographic scenario of speciation events in these two anuran genera. Based on two alternative, mutually exclusive calibrations of the Iberian-African split within Pelobates (Pb. cultripes and Pb. varaldii), the disjunction of the Betic Cordillera ca. 14-16 million years ago (mya), and the end of the Messinian Salinity crisis 5.33 mya, we inferred alternative scenarios for species evolution within both genera applying regression-based dating and Bayesian molecular dating. Pelobates and Pelodytes are both monophyletic genera. Interspecifi c relationships among spadefoot toads are poorly resolved, and only an Iberian-African Pb. cultripes/Pb. varaldii clade consistently emerges from our analyses. An evolutionary scenario based on the Messinian divergence of African and Iberian Pelobates lineages becomes plausible in the light of geological and paleontological data. Consequently, Pelobates species are likely to have originated from the Miocene. Speciation around the Oligocene/Miocene boundary is inferred for the Iberian-Caucasian Pelodytes, and a Messinian divergence has to be invoked to explain intraspecific diversifi cation of Iberian parsley frogs. There is indication that the different Pb. syriacus lineages may not form a monophylum.
References
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Journal ArticleDOI
Genome size and GC‐percent in vertebrates as determined by flow cytometry: The triangular relationship
TL;DR: In this paper, the authors used DNA flow cytometry to determine the genome size and GC-percent of 154 vertebrate species and found that the overall distribution of points is not linear but triangular, whereas with an increase in genome size the lower limit for GC- percent is elevated, gradually approaching the upper limit.
Journal Article
Nucleotype and cell size in vertebrates: a review.
TL;DR: In Vertebrates, genome size would exert a real nucleotypic influence on cell size and genome sizes and cell morphometric parameters seem to be involved in the regulation of cell metabolism.
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Genome elimination in diploid and triploid Rana esculenta males: cytological evidence from DNA flow cytometry.
TL;DR: Data are presented showing that elimination of the R. ridibunda genome occurs in hybridogenetic males from certain populations, and the process of elimination can be visualized in triploid males by using DNA flow cytometry to identify cells in the special phase of the spermatogonial cell cycle that is termed the E phase.
Journal ArticleDOI
Quantitative Variations in the Nuclear DNA and Phylogenesis of the Amphibia
TL;DR: The results emphasize the existence of extensive interspecific variations in the amounts of DNA in the Amphibia, as well as certain facts of possible systematic interest: the Urodela possess greater amounts of nuclear DNA than most of the Apoda and the Anura.
Journal ArticleDOI
Trends in nuclear DNA content among amphibians and reptiles
TL;DR: Results indicate that two or more populations should be sampled to ensure a more accurate estimate of intraspecific variation in amphibians and reptiles.