Journal ArticleDOI
Crystallisation and preliminary crystallographic analysis of P1 nuclease from Penicillium citrinum.
TLDR
P1 nuclease, a zinc-dependent single-strand specific endonuclease from Penicillium citrinum, has been crystallized in three different space groups using either ammonium sulphate or polyethylene glycol 4000 as the precipitating agent.About:
This article is published in Journal of Molecular Biology.The article was published on 1990-09-20. It has received 68 citations till now. The article focuses on the topics: Penicillium citrinum & Crystal structure.read more
Citations
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Journal ArticleDOI
Crystal structure of Penicillium citrinum P1 nuclease at 2.8 A resolution.
TL;DR: P1 nuclease from Penicillium citrinum is a zinc dependent glyco‐enzyme which cleaves single‐stranded RNA and DNA into 5′‐mononucleotides and a cleavage mechanism is proposed involving nucleophilic attack by a Zn activated water molecule.
Journal ArticleDOI
New perspective on zinc biochemistry: cocatalytic sites in multi-zinc enzymes.
Bert L. Vallee,David S. Auld +1 more
Journal ArticleDOI
Single-strand-specific nucleases
Neelam A. Desai,Vepatu Shankar +1 more
TL;DR: The detection, purification, characteristics, structure-function correlations, biological role and applications of single-strand-specific nucleases are reviewed.
Journal ArticleDOI
Dimetallic hydrolases and their models.
TL;DR: This work has shown that the competitive binding of hydroxyl and phosphate substrates has been demonstrated with dizinc and trizinc complexes, and confirmed the role of Fe3+/M2+ purple acid phosphatase in phosphate hydrolysis.
Journal ArticleDOI
Single-Strand-Specific Nucleases
S U Gite,Vepatu Shankar +1 more
TL;DR: Single-strand-specific nucleases, their purification, characteristics, biological role, and applications have been reviewed.
References
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Journal ArticleDOI
Solvent content of protein crystals.
TL;DR: An analysis of the solvent content of 116 different crystal forms of globular proteins found that in many cases this range will be sufficiently restrictive to enable the probable number of molecules in the crystallographic asymmetric unit to be determined directly from the molecular weight of the protein and the space group and unit cell dimensions of the crystal.
Book ChapterDOI
Resolution of phase ambiguity in macromolecular crystallography.
TL;DR: The method demonstrates a remarkable effectiveness for overcoming the phase ambiguity problem that has the major obstacle in the use of SIR or SAS data alone for macromolecular studies.
Journal ArticleDOI
High-resolution (1.5 Å) crystal structure of phospholipase C from Bacillus cereus
Edward Hough,Lars Kristian Hansen,Bjørn Birknes,Knut Jynge,Sissel Hansen,Asbjørn Hordvik,Clive Little,Eleanor J. Dodson,Zygmunt S. Derewenda +8 more
TL;DR: The three-dimensional structure of B. cereus PLC is reported and it is shown that the enzyme is an all-helix protein belonging to a novel structural class and contains, at least in the crystalline state, three Zn2+ in the active site.
Journal ArticleDOI
Synthesis and configurational analysis of a dinucleoside phosphate isotopically chiral at phosphorus. Stereochemical course of Penicillium citrinum nuclease P1 reaction
TL;DR: It is shown that the stereochemical course of the hydrolytic reaction catalyzed by nuclease P1 from Penicillium citrum proceeds with inversion of configuration at phosphorus and therefore probably does not involve the participation of a covalent enzyme intermediate.
Journal ArticleDOI
A reciprocal-space method for calculating a molecular envelope using the algorithm of B.C. Wang
TL;DR: In this paper, a method was described to determine the molecular envelope from an isomorphous replacement phased electron density map using the reciprocal-space equivalent of B. C. Wang's algorithm.
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