Definition of a Continuous Human Cell Line Derived from Neuroblastoma

TLDR: A continuous hyperdiploid human cell line, IMR-32, was derived from neuroblastoma tissue and proved a relatively poor host for virus replication, particularly for representative viruses of two of the human enterovirus groups.

Abstract: Summary A continuous hyperdiploid human cell line, IMR-32, was derived from neuroblastoma tissue. At least two morphologically distinct cell types comprise the population of IMR-32. These two cell types have the same characteristic chromosomal constitution and hyperdiploid stem line number, indicating a common clonal origin. One of the cell types, generally rarely observed because it occurs in minute numbers, appears to differentiate from the major or predominating cell. Marked ultrastructural differences were noted between the 2 cell types. The cell line proved a relatively poor host for virus replication, particularly for representative viruses of two of the human enterovirus groups. The electrophoretically more mobile lactic dehydrogenase isozymes of IMR-32 were retained after extended subcultivation.