Journal ArticleDOI
Denaturation of globular proteins by guanidine thiocyanate I. Optical rotation in aqueous guanidine thiocyanate solutions
TLDR
It is found that with increasing salt concentration the optical rotation of protein solutions becomes increasingly negative, reaches a minimum between 2.5 and 3.5 M, and then increases slowly, leading to the conclusion that in 4.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1971-09-28. It has received 17 citations till now. The article focuses on the topics: Denaturation (biochemistry) & Globular protein.read more
Citations
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Journal ArticleDOI
Detection of four apple viruses by multiplex RT-PCR assays with coamplification of plant mRNA as internal control.
TL;DR: Two multiplex RT-PCR assays with specific coamplification of plant mRNA as an internal control from total nucleic acids are described for the parallel detection of Apple chlorotic leaf spot virus,Apple stem pitting virus, Apple mosaic virus and Apple stem grooving virus.
Journal ArticleDOI
Estimation of the free energy of stabilization of ribonuclease A, lysozyme, alpha-lactalbumin, and myoglobin.
Faizan Ahmad,C C Bigelow +1 more
TL;DR: The denaturation of ribonuclease A, lysozyme alpha-lactalbumin, and myoglobin by urea, Guanidine hydrochloride, and guanidine thiocyanate has been followed with the use of difference spectral measurements and the free energy of stabilization (delta GH2OD) has been determined.
Journal ArticleDOI
Folding of beta-sandwich proteins: three-state transition of a fibronectin type III module.
Ernesto Cota,Jane Clarke +1 more
TL;DR: This is the first demonstration of three‐state folding kinetics for a fnIII domain and it is shown that a homologous domain from human tenascin (TNfn3) folds by a two‐state mechanism, but this does not necessarily indicate that the two proteins fold by different folding pathways.
Journal ArticleDOI
Guanidine hydrochloride and the circular dichroism of random coil polypeptides
TL;DR: In the case of insulin, evidence is given to suggest that this arises from intra-chain interactions, rather than the intrinsic optical activity of the disulphide chromophores, which seems likely that composition-dependence of the distribution of backbone dihedral angles is responsible for the variation.
Journal ArticleDOI
Liquid-phase hybridization and capture of hepatitis B virus DNA with magnetic beads and fluorescence detection of PCR product.
TL;DR: PCR of hepatitis B virus deoxyribonucleic acid was used to demonstrate how easy, versatile, and time-saving this method is without centrifugation.
References
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Journal ArticleDOI
Proteins as Random Coils. I. Intrinsic Viscosities and Sedimentation Coefficients in Concentrated Guanidine Hydrochloride
Journal ArticleDOI
On the conformational stability of globular proteins - the effects of various electrolytes and nonelectrolytes on the thermal ribonuclease transition
Peter H. von Hippel,K Y Wong +1 more
TL;DR: Physicochemical instrumental analysis methods used in the study include polarimetry, viscometry, ultraviolet (UV) spectroscopy, short column sedimentation (weight average molecular weights) and spectrophotometry and interferometry were used to characterize the bovine pancreatic ribonuclease.