Development of a standardized subgrouping scheme for Legionella pneumophila serogroup 1 using monoclonal antibodies.
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A panel of monoclonal antibodies to Legionella pneumophila serogroup 1 and a subclassification scheme were developed in a collaborative project among three laboratories and a standard protocol for the indirect fluorescent antibody procedure was developed.Abstract:
A panel of monoclonal antibodies to Legionella pneumophila serogroup 1 and a subclassification scheme were developed in a collaborative project among three laboratories. The seven most useful monoclonal antibodies were selected from three previously developed panels on the basis of indirect fluorescent antibody patterns with 83 strains of L. pneumophila serogroup 1 that were obtained from widely distributed geographic locations. The isolates were divided into 10 major subgroups on the basis of reactivity patterns that can be readily reproduced in any laboratory and are not subject to major inconsistencies of interpretation of staining intensity. A standard protocol for the indirect fluorescent antibody procedure was also developed.read more
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References
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Journal ArticleDOI
Legionnaires' Disease: Description of an Epidemic of Pneumonia
David W. Fraser,T R Tsai,Walter A. Orenstein,W E Parkin,H J Beecham,R G Sharrar,Harris Jc,George F. Mallison,S M Martin,Joseph E. McDade,C C Shepard,P S Brachman +11 more
TL;DR: An explosive, common-source outbreak of pneumonia caused by a previously unrecognized bacterium affected primarily persons attending an American Legion convention in Philadelphia in July, 1976, killing 29 people, and epidemiologic analysis suggested that exposure may have occurred in the lobby of the headquarters hotel or in the area immediately surrounding the hotel.
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Legionnaires' disease: isolation of a bacterium and demonstration of its role in other respiratory disease.
Joseph E. McDade,C C Shepard,David W. Fraser,Theodore R. Tsai,Martha A. Redus,Walter R. Dowdle +5 more
TL;DR: It is concluded that Legionnaires' disease is caused by a gram-negative bacterium that may be responsible for widespread infection.
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Ecological distribution of Legionella pneumophila.
TL;DR: The data suggest that Legionella pneumophila is part of the natural aquatic environment and that the bacterium is capable of surviving extreme ranges of environmental conditions, and demonstrate the effectiveness of the direct fluorescent-antibody technique for detecting L. pneumophILA in natural aquatic systems.
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An outbreak of Legionnaires' disease associated with a contaminated air-conditioning cooling tower
TL;DR: A significant association was demonstrated between acquisition of Legionnaires' disease and prior hospitalization in those areas of Hospital A that received ventilating air from air intakes near the auxiliary cooling tower.
Journal ArticleDOI
Legionnaires' disease in a transplant unit: isolation of the causative agent from shower baths.
J. O'h. Tobin,J. O'h. Tobin,J. O'h. Tobin,M.S. Dunnill,M.S. Dunnill,M.S. Dunnill,Michael E. French,Michael E. French,Michael E. French,P.J. Morris,P.J. Morris,P.J. Morris,J. Beare,J. Beare,J. Beare,S.P. Fisher-Hoch,S.P. Fisher-Hoch,S.P. Fisher-Hoch,R.G. Mitchell,R.G. Mitchell,R.G. Mitchell,M.F. Muers,M.F. Muers,M.F. Muers +23 more
TL;DR: Patients in a transplant unit diagnosed in two patients having occupied the same postoperative cubicle shortly before onset of their illnesses had Legionnaires' disease, and water taken from the cubicle shower bath and from other showers in the unit was treated with chlorine.