Journal ArticleDOI
Different template specificities of phage T3 and T7 RNA polymerases.
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TLDR
Chamberlin et al. have separated the gene 1 product from the host enzyme in Escherichia coli and show that gene 1 codes for an entirely new RNA polymerase whioii transcribes T7 DNA efficiently and exclusively compared with other templates.Abstract:
THE transcription of the late genes of phage T7 depends on the function of gene 1 of the phage1. Chamberlin et al.2 have separated the gene 1 product from the host enzyme in Escherichia coli and have shown that gene 1 codes for an entirely new RNA polymerase whioii transcribes T7 DNA efficiently and exclusively compared with other templates. Summers and Siegel3 have shown that this enzyme transcribes the late region of the T7 genome.read more
Citations
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A study in evolution: the DNA base sequence homology between coliphages T7 and T3.
Ronald W. Davis,Richard W. Hyman +1 more
TL;DR: The observation that the fraction of the heteroduplex observed as double-stranded decreases with an increase in denaturing conditions indicates that the DNA's of T7 and T3 have extensive sequences of partial homology.
Journal ArticleDOI
Characterization of T7-specific ribonucleic acid polymerase. 1. General properties of the enzymatic reaction and the template specificity of the enzyme.
Michael J. Chamberlin,Janet Ring +1 more
TL;DR: It is suggested that T7 RNA polymerase requires a specific promoter site on DNA for effective transcription; this site is different from that used by bacterialRNA polymerase and may be rich in cytosine and thymidine residues.
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Transposon end compositions and methods for modifying nucleic acids
TL;DR: In this paper, a transposase and transposon end are used for generating extensive fragmentation and 5-tagging of double-stranded target DNA in vitro, and then using a DNA polymerase for generating 5-and 3-tagged single-standed DNA fragments without performing a PCR amplification reaction.
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Modified nucleosides, nucleotides, and nucleic acids, and uses thereof
TL;DR: The present disclosure provides modified nucleosides, nucleotides, and nucleic acids, and methods of using them as discussed by the authors, as well as a detailed discussion of their properties.
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Engineered nucleic acids and methods of use thereof
TL;DR: In this paper, compositions and methods for delivering biological moieties such as modified nucleic acids into cells to modulate protein expression are provided. But they do not consider the use of modified messenger RNAs, which are useful to treat or prevent diseases, disorders or conditions.
References
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Journal ArticleDOI
Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels
Journal ArticleDOI
Factor stimulating transcription by RNA polymerase.
TL;DR: A protein component usually associated with RNA polymerase can be separated from the enzyme by chromatography on phosphocellulose, and the polymerase is unable to transcribe T4 DNA unless this factor is added back.
Journal ArticleDOI
A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.
TL;DR: A method for the purification of Escherichia coli DNA-dependent RNA polymerase which is rapid, reproducible, high in yield, and able to handle preparations using from 1 g to 3 kg of cells is described.
Journal ArticleDOI
New RNA polymerase from Escherichia coli infected with bacteriophage T7.
TL;DR: A new T7-specific RNA polymerase is found in T7 phage-infected cells and provides a direct explanation for the pleiotropic control of late T7 functions exerted by gene I.
Journal ArticleDOI
Inhibition of dna-dependent rna synthesis by rifamycins
TL;DR: The results indicate that the incorporation of 3H-CMP into acid insoluble fraction by RNA polymerase of E. coli was markedly inhibited by both anti biotics at a concentration as low as 0.1 jum.