Journal ArticleDOI
New RNA polymerase from Escherichia coli infected with bacteriophage T7.
TLDR
A new T7-specific RNA polymerase is found in T7 phage-infected cells and provides a direct explanation for the pleiotropic control of late T7 functions exerted by gene I.Abstract:
A new T7-specific RNA polymerase is found in T7 phage-infected cells It is the product of T7 gene I and is physically and biochemically distinct from the host cell RNA polymerase The synthesis of T7 RNA polymerase provides a direct explanation for the pleiotropic control of late T7 functions exerted by gene Iread more
Citations
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Book ChapterDOI
Use of T7 RNA polymerase to direct expression of cloned genes.
Journal ArticleDOI
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
F W Studier,B A Moffatt +1 more
TL;DR: A gene expression system based on bacteriophage T7 RNA polymerase has been developed and high levels of accumulation suggest that the RNAs are relatively stable, perhaps in part because their great length and/or stem-and-loop structures at their 3' ends help to protect them against exonucleolytic degradation.
Journal ArticleDOI
Analysis of bacteriophage T7 early RNAs and proteins on slab gels
TL;DR: The RNAs and proteins specified by five early genes of bacteriophage T7 have been identified by electrophoresis on sodium dodecyl sulfate, polyacrylamide gels using a slab gel system in which 25 samples can be run simultaneously and then dried for autoradiography.
Journal ArticleDOI
Vectors for selective expression of cloned DNAs by T7 RNA polymerase
TL;DR: Plasmid vectors are described that allow cloning of target DNAs at sites where they will be minimally transcribed by Escherichia coli RNA polymerase but selectively and actively transcribing by T7 RNA polymerases, in vitro or in E. coli cells.
Journal ArticleDOI
Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system.
TL;DR: Bacteriophage T7 lysozyme can reduce basal activity from an inducible gene for T7 RNA polymerase and allow relatively toxic genes to be established in the same cell under control of a T7 promoter.
References
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Journal ArticleDOI
A Method for Determining the Sedimentation Behavior of Enzymes: Application to Protein Mixtures
Robert G. Martin,Bruce N. Ames +1 more
TL;DR: Sucrose gradient centrifugation is found to be a suitable method for determining sedimentation coefficients of enzymes in protein mixtures and the sedimentation behavior of several of the enzymes in the pathway of histidine biosynthesis in S. typhimurium has been determined.
Journal ArticleDOI
Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels
Journal ArticleDOI
A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane
David Gillespie,Sol Spiegelman +1 more
TL;DR: An improved method for the formation of DNA—RNA hybrids is described, quantitatively more certain than annealing in liquid, since the competing DNA renaturation reaction is suppressed.
Journal ArticleDOI
A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. Coli I. Purification and characterization of alkaline phosphatase
A. Garen,C. Levinthal +1 more
TL;DR: A procedure for purification of alkaline phosphatase from E. coli is described, and several physical-chemical propetiies of the purified enzyme are reported.
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Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
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