Journal ArticleDOI
Differentiation in Callus Cultures of Leaf of Two Species of Trigonella
Basantak Sen,Sukumar Gupta +1 more
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Callusing of leaf segment of Trigonella corniculata and Trigonelta foenum graecum was observed on Murashige and Skoog's medium and White's medium supplemented with naphthalene acetic acid and coconut milk, and higher differentiation rate was observed at low irradiance than at high irradiance or in complete darkness.Abstract:
Callusing of leaf segment of Trigonella corniculata L. and Trigonelta foenum graecum L. was observed on Murashige and Skoog's (MS) medium and White's (W) medium supplemented with naphthalene acetic acid (NAA; 0.5 mg/1) and coconut milk (15% v/v). Root, shoot and development of isolated leaf occurred in the calli of both the species on MS medium containing NAA amd coconut milk. Omission of coconut milk with subsequent addition of casein hydrolysale (CH) increased the number of differentiated organs per callus. In both the materials higher differentiation rate was observed at low irradiance than at high irradiance or in complete darkness. Initially the cells were diploid but at the 5th passage the calli became mixoploid wilh predominantly diploid cell populations. The differentiated organs were diploid.read more
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Journal ArticleDOI
In vitro manipulation and propagation of medicinal plants
TL;DR: An overview of the regeneration of medicinal plants by direct and indirect organogenesis and by somatic embryogenesis from various types of explants is presented, and the use of these techniques combined with other biotechnological approaches to improve medicinal plants through somaclonal variation and genetic transformation is reviewed.
Book ChapterDOI
Biotechnology of the Micropropagation of Medicinal and Aromatic Plants
TL;DR: Among medicinal plants most research studies deal with the production and biotransformation of pharmacologically active principles by tissue culture, which includes plants which contain compounds useful in medicine and pharmacy.
Journal ArticleDOI
Mesophyll protoplasts of fenugreek (Trigonella foenumgraecum): Isolation, culture and shoot regeneration.
TL;DR: High yields of mesophyll protoplasts were obtained after treatment of leaves of Trigonella foenumgraecum, a forage legume, with purified cellulase and addition of glutamine and asparagine to the medium was found essential for rapid cell division, callus growth and differentiation.
Book ChapterDOI
Plant Growth Substances in Commercial Uses of Tissue Culture
TL;DR: Manipulations involving auxin and cytokinin provisions continue to serve as the basis of nearly all of today’s plant tissue culture.
References
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Journal ArticleDOI
Root Formation of Pea Cuttings in Relation to the Irradiance of the Stock Plants
TL;DR: The results indicate that the nutritional status of the stock plant is an important factor for root formation in the cutting and light may influence the production of inhibitors which directly or indirectly affect root formation.
Journal ArticleDOI
Morphogenesis in Tissue Cultures of Different Organs of Nigella sativa
S. Banerjee,Sukumar Gupta +1 more
TL;DR: From all the three types of calli, roots were differentiated and shoot development occurred in stem and leaf calli only after the omission of first coconut milk and then of auxin from the basal medium.
Journal ArticleDOI
Tissue culture ofNigella sativa I. The behaviour of nucleus
TL;DR: In Kallusgewebekulturen von Nigella sativa nimmt mit der Zeit der Anteil der diploiden Zellen ab, wahrend sich die Anteile von aneupliden, von 2n abweichenden Zelle vermehren.
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