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Journal ArticleDOI

DNA Damage in Vicia faba by Exposure to Agricultural Soils from Tlaxcala, Mexico.

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TLDR
The agricultural soils of Tlaxcala, Mexico have greater genotoxic capacity than the organic extracts, suggesting a potential risk to biota that depends upon this ecosystem.
Abstract
The aim of this research was to quantify some POPs, such as p,p′ DDT, p,p′ DDE, and PCBs in agricultural soils of Tlaxcala, Mexico and evaluate their capacity for eliciting DNA damage, using Vicia faba as bioindicator. The values of ΣDDTs and ΣPCBs ranged from 8–24 to 118–26,983 µg/kg, respectively. The samples T1 (HQ = 9.3) and T2 (HQ = 53.9) showed concentrations of ΣPCBs higher than Canadian guidelines (SQGE = 500 µg/kg). The genotoxicity testing produced percentages of DNA fragmentation higher than negative control and statistically significant (p < 0.05), both in agricultural soils and organic extracts. The soils T2, T3, N4, and N5 showed a DICA from 2.6 to 3.1 times, statistically higher (p < 0.05) than negative control. In general, the agricultural soils have greater genotoxic capacity than the organic extracts, suggesting a potential risk to biota that depends upon this ecosystem.

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Citations
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Journal ArticleDOI

Heavy metal accumulation and genotoxic effect of long-term wastewater irrigated peri-urban agricultural soils in semiarid climate.

TL;DR: In this paper, physicochemical properties, genotoxicity (Vicia faba micronucleus test), total and available (CaCl2-extractable) concentrations of Cr, Pb, Cu, Zn, Co and Cd in eight soils of peri-urban farms irrigated with wastewater were examined to evaluate their accumulation.
Journal ArticleDOI

Malathion and dithane induce DNA damage in Vicia faba

TL;DR: RAPD assays can be useful in determining genotoxicity of pesticides in V. faba and other crops along with other quantitative parameters, and DNA damage observed by the cytogenetic endpoints and comet assay corroborated with RAPD-PCR analysis.
Journal ArticleDOI

Genotoxicity Assessment of Volatile Organic Compounds in Landfill Gas Emission Using Comet Assay in Higher Terrestrial Plant

TL;DR: Comet assay of Pothos can be used in differentiating DNA fragmentation against very low levels of BTEX in the atmosphere and is recommended for genotoxicity assessment of a low BTEX contaminated atmosphere.
Journal ArticleDOI

Genotoxicity of Organic contaminants in the soil: A review based on bibliometric analysis and methodological progress.

TL;DR: Zhang et al. as mentioned in this paper summarized the manifestations and influencing factors of genotoxicity of organic contaminants to soil organisms, the main damage mechanisms, and the most commonly utilized testing methods.
References
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Journal ArticleDOI

Comet assay: a reliable tool for the assessment of DNA damage in different models

TL;DR: This review is an attempt to comprehensively encase the use of Comet assay in different models from bacteria to man, employing diverse cell types to assess the DNA-damaging potential of chemicals and/or environmental conditions.
Book

Organic Pollutants : An Ecotoxicological Perspective

C.H. Walker
TL;DR: A companion to the highly praised Principles of Ecotoxicology has been published in this paper, which covers organic pollutants in greater depth and detail than has been covered in a textbook before and offers a concise source of information on these compounds at a level suitable for senior undergraduates and postgraduates.
Journal ArticleDOI

Lead-induced DNA damage in Vicia faba root cells: potential involvement of oxidative stress.

TL;DR: The results clearly indicate that lead induced DNA fragmentation in a dose-dependant manner with a maximum effect at 10μM, which reveals the major role of reactive oxygen species (ROS) in the genotoxicity of lead.
Journal ArticleDOI

Occurrence of brominated flame retardants (BFRs), organochlorine pesticides (OCPs), and polychlorinated biphenyls (PCBs) in agricultural soils in a BFR-manufacturing region of North China.

TL;DR: Deca-BDE was shown to be persistent in the soil and displayed a spatial distribution opposite to that of BFRs, which was concentrated in the industrial zone.
Journal ArticleDOI

A fast comet assay variant for solid tissue cells. The assessment of DNA damage in higher plants

TL;DR: The use of instantaneously isolated nuclei simplifies and expands the use of this technique to any eukaryotic cell from solid tissues, without altering their later DNA staining by ethidium bromide.
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