DNA hydration studied by neutron fiber diffraction.
Watson Fuller,V.T. Forsyth,Arumugam Mahendrasingam,Paul Langan,W. J. Pigram,Sax A. Mason,C. C. Wilson +6 more
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TLDR
Analysis of neutron high angle fiber diffraction studies to investigate the location of water around the deoxyribonucleic acid (DNA) double-helix emphasizes the importance of viewing DNA, water and ions as a single system with specific interactions between the three components.Abstract:
The development of neutron high angle fiber diffraction to investigate the location of water around the deoxyribonucleic acid (DNA) double-helix is described. The power of the technique is illustrated by its application to the D and A conformations of DNA using the single crystal diffractometer, D19, at the Institut Laue-Langevin, Grenoble and the time of flight diffractometer, SXD, at the Rutherford Appleton ISIS Spallation Neutron Source. These studies show the existence of bound water closely associated with the DNA. The patterns of hydration in these two DNA conformations are quite distinct and are compared to those observed in X-ray single crystal studies of two-stranded oligodeoxynucleotides. Information on the location of water around the DNA double-helix from the neutron fiber diffraction studies is combined with that on the location of alkali metal cations from complementary X-ray high angle fiber diffraction studies at the Daresbury Laboratory SRS using synchrotron radiation. These analyses emphasize the importance of viewing DNA, water and ions as a single system with specific interactions between the three components and provide a basis for understanding the effect of changes in the concentration of water and ions in inducing conformational transitions in the DNA double-helix.read more
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Intracrystalline deuteration of native cellulose
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X-ray and neutron diffraction studies of the structure of PEEK
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Abstract: Abstract Neutron fibre diffraction has been used to determine the orientation of phenyl rings with respect to the polymer backbone in poly (aryl-ether-ether-ketone) (PEEK). Results obtained from this study indicate that the orientation of the phenyl ring differs by 7° from the previously accepted value based on X-ray fibre diffraction studies.
References
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Structure of a B-DNA dodecamer: III. Geometry of hydration
TL;DR: The dodecamer d(cpGpCpGPGpApApApTpTpPTpC pGpG pCpCPG pG pApAp ApApT pTp TpG PGp CpG-C-G-A A-A-T-T T-T, C-Gg-Cc-Cg-Gp Gp CcG-G, GpGgp CgGpP GpCcG p Cgp G
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Left-handed DNA helices
Struther Arnott,Rengaswami Chandrasekaran,D. L. Birdsall,A.G.W. Leslie,A.G.W. Leslie,R. L. Ratliff,R. L. Ratliff +6 more
TL;DR: X-ray diffraction of orientated fibres in which poly d(GC) has either the well known right-handed conformations or a new left-handed helical conformation demonstrates that the novel structure is accessible to any DNA segment with an alternating purine–pyrimidine base sequence.
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The molecular configuration of deoxyribonucleic acid: II. Molecular models and their fourier transforms
Robert Langridge,D.A. Marvin,W. E. Seeds,H. R. Wilson,C. W. Hooper,M. H. F. Wilkins,L. D. Hamilton +6 more
TL;DR: In this article, the Fourier transform of the structure has been calculated, and comparison with the X-ray data described in Part I shows that the model needs modifying, and various models were built and adjusted until reasonable agreement was obtained between the Fouriers transform, averaged by rotation about the helix axis, and the observed 2D intensity data.
Journal ArticleDOI
The molecular configuration of deoxyribonucleic acid
TL;DR: In this paper, an analysis has been made of the oriented crystalline pattern given by fibres of the lithium salt (LiDNA) at 66% relative humidity, which is known as the B type and exists in unfixed cell nuclei.