Effect of specific binding of human albumin, fibrinogen, and immunoglobulin G on surface characteristics of bacterial strains as revealed by partition experiments in polymer phase systems.
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There was a correlation between the binding of radiolabled human proteins to the bacterial strains and the effect of human proteins on the partition of the bacteria in the phase systems, suggesting that these types of bacteria-protein interactions may play an important role in modulating host-parasite relationships.Abstract:
Four strains of gram-positive cocci with different combinations of positive binding of human proteins were investigated with respect to changes in physicochemical surface properties after specific protein binding. Staphylococcus aureus Cowan I, two group A beta-hemolytic streptococci, and one group G streptococcal strain were studied; they represented three different combinations of reactivity for human serum albumin, human immunoglobulin G, and fibrinogen. Using single-tube partition of bacterial cells in a dextran-polyethylene glycol system of constant polymer concentration but varying ionic compositions, it was possible to detect changes in the partition of bacteria after specific protein binding. There was a correlation between the binding of radiolabled human proteins to the bacterial strains and the effect of human proteins on the partition of the bacteria in the phase systems. Thus, the specific binding of proteins to the bacteria changes their physicochemical surface properties. These types of bacteria-protein interactions may play an important role in modulating host-parasite relationships.read more
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References
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Arne Forsgren,John Sjöquist +1 more
TL;DR: Protein A was isolated from S. aureus and shown to precipitate about 45% of a pooled normal human γG preparation, but this reaction is not a true antigen-antibody reaction.
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Phagocytosis as a Surface Phenomenon
TL;DR: The aim of this monograph is to demonstrate the efforts towards in-situ applicability of the multi-Liquid Contact Angle Method in relation to surface-to-surface contact Angle Matching.
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A surface component in group A, C, and G streptococci with non-immune reactivity for immunoglobulin G.
TL;DR: Experiments using isolated myeloma globulins representing the four IgG subclasses indicated that streptococci might react not only with IgG1-, IgG2-, and IgG4-like protein A but also with Igg3 molecules.