Efficiency of Airborne Sample Analysis Platform (ASAP) bioaerosol sampler for pathogen detection
TLDR
The efficacy of the Airborne Sample Analysis Platform (ASAP) 2800Bioaerosol sampler to collect representative samples of air and identify specific viruses suspended as bioaerosols is investigated and results support the further development of ASAP for bioaerOSol pathogen detection.Abstract:
The threat of bioterrorism and pandemics has highlighted the urgency for rapid and reliable bioaerosol detection in different environments. Safeguarding against such threats requires continuous sampling of the ambient air for pathogen detection. In this study we investigated the efficacy of the Airborne Sample Analysis Platform (ASAP) 2800 bioaerosol sampler to collect representative samples of air and identify specific viruses suspended as bioaerosols. To test this concept, we aerosolized an innocuous replication-defective bovine adenovirus serotype 3 (BAdV3) in a controlled laboratory environment. The ASAP efficiently trapped the surrogate virus at 5 × 10(3) plaque-forming units (p.f.u.) [2 × 10(5) genome copy equivalent] concentrations or more resulting in the successful detection of the virus using quantitative PCR. These results support the further development of ASAP for bioaerosol pathogen detection.read more
Citations
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Journal ArticleDOI
Bioaerosol sampling: Classical approaches, advances, and perspectives
TL;DR: In this paper, the authors describe the essential and integral part of any bioaerosol investigation, including sampling and analysis of bio-aerosols in terms of their sizes, species, biological properties, and requirements.
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DNA metabarcoding uncovers fungal diversity of mixed airborne samples in Italy
Elisa Banchi,Claudio G. Ametrano,David Stanković,Pierluigi Verardo,Olga Moretti,Francesca Augusta Gabrielli,Stefania Lazzarin,Maria Francesca Borney,Francesca Tassan,Mauro Tretiach,Alberto Pallavicini,Lucia Muggia +11 more
TL;DR: The results prove that the metabarcoding analysis is a promising approach to increases quality and sensitivity of the aerobiological monitoring and the laboratory and bioinformatic workflow implemented here is now suitable for routine, high-throughput, regional analyses of airborne fungi.
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Fingerprinting outdoor air environment using microbial volatile organic compounds (MVOCs) – A review
Sonia Garcia-Alcega,Zaheer Ahmad Nasir,Robert M. W. Ferguson,Corinne Whitby,Alex J. Dumbrell,Ian Colbeck,Diogo Gomes,Sean Tyrrel,Frederic Coulon +8 more
TL;DR: In this article, the most suitable techniques for sampling and analysing microbial volatile organic compounds (MVOCs) in outdoor environments are reviewed and the need for further studies on MVOCs from outdoor environments including background levels is highlighted.
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Methods for Early Detection of Microbiological Infestation of Buildings Based on Gas Sensor Technologies
Monika Garbacz,Agnieszka Malec,Sylwia Duda-Saternus,Zbigniew Suchorab,Łukasz Guz,Grzegorz Łagód +5 more
TL;DR: In this article, a review of the problem of microbiological infestation of buildings was discussed, with special attention drawn to the rapid-early detection methods based on gas sensor arrays, and the harmful effects on human health caused by the microbiological contamination were discussed with a short review of particular groups of microorganisms causing sick building syndrome.
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Sensors and Analytical Technologies for Air Quality: Particulate Matters and Bioaerosols.
TL;DR: The differential air sampling and detection challenges for viral aerosols relative to bacterial and fungal aerosols using Influenza A and SARS-CoV-2 virus are highlighted.
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