Journal ArticleDOI
Electron microscopic study of Trypanosoma cruzi in thin sections of infected tissue cultures and of blood-agar forms.
TLDR
In the adult form of the Trypanosoma cruzi, a great vacuole is formed near the posterior tip of the trypanosome and is often dislodged by the size of the vacuoles as discussed by the authors.Abstract:
Thin sections of Trypanosoma cruzi in tissue cultures and from blood agar medium have been examined with the electron microscope.In the leishmania and crithidia forms of the parasite a neat separation of kinetonucleus and blepharoplast has been obtained and these structures are described. The blepharoplast is the row of basal corpuscles which give origin to the axial fibres of the flagellum.Division of the parasite seems to begin in the basal corpuscles; it is followed by binary division of the kinetonucleus and only later by fission of nucleus and cytoplasm.During the development of the leishmania into the crithidia form, the kinetonucleus and basal bodies are dislocated towards the posterior half of the trypanosome. The migration of these two structures and the consequent lengthening of the flagellum causes the surface membrane of the flagellum to be pushed inwards to form a deep invagination, so that the flagellum comes to the surface of the body and is separated from it along its whole length by its own sheath.In the adult form of the parasite a great vacuole is formed near the posterior tip of the trypanosome. It has no definite structure in the electron microscope, is transparent and seems to be filled with a liquid content. The basal corpuscles are situated in this region near the wall of the vacuole. The typical structure of the kinetonucleus could not be identified with certainty in these forms; it seems to be altered by the formation of the vacuole.The vacuole is crossed by a fibre system which comes from the body of the trypanosome and is often dislodged by the size of the vacuole. This fibre system ends in a sharply pointed process of varying length.The cost of reproduction of figures was defrayed by the Instituto de Biofisica da Universidade do Brasil.read more
Citations
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Book ChapterDOI
Chagas' disease and Chagas' syndromes: the pathology of American trypanosomiasis.
TL;DR: American trypanosomiasis shows a very peculiar pathology of homeostasis of the human organism, and represents even today a new realm in pathology, which becomes understandable through Cannon's law of denervation.
Journal ArticleDOI
Trypanosoma cruzi: mechanism of entry and intracellular fate in mammalian cells.
TL;DR: Electron microscopic observations indicated the uptake by phagocytosis of both forms into mouse peritoneal macrophages and of trypomastigotes and transition forms into other cultured cell types, indicating that protease-sensitive structures on the macrophage plasma membrane mediate ingestion.
Book ChapterDOI
Cell Biology of Trypanosoma cruzi
TL;DR: This chapter reviews some aspects of the cell biology of Trypanosoma cruzi, giving emphasis to those aspects related to the ultrastructure of pathogenic protozoa.
Journal ArticleDOI
Ultrastructure of Trypanosoma lewisi: Flagellum, Microtubules, and the Kinetoplast
W. A. Anderson,R. A. Ellis +1 more
TL;DR: The ultrastructure of Trypanosoma lewisi is re-examined with particular reference to the flagellum and pellicle, indicating that this organelle is probably primarily concerned with the ontogenesis of mitochondria, and several mitochondria extend into the cytoplasm of the cell.
Journal ArticleDOI
Pathogenesis of Chagas' Disease: Parasite Persistence and Autoimmunity
TL;DR: The phenotyping of the lesions revealed that cytotoxic CD45, CD8+ γδ, and CD8α+ T lymphocytes carry out the rejection of the chicken heart, suggesting that the inflammatory cardiomyopathy of Chagas' disease is a genetically driven autoimmune disease.
References
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Journal ArticleDOI
A study of fixation for electron microscopy
TL;DR: Fixation experiments with buffered OsO4 solutions have shown that the appearance of the fixed cells is conditioned by the pH of the fixative, and the quality of fixation can be materially improved by buffering the OsO 4 solutions at pH 7.3-7.5 with acetate-veronal buffer.
Journal ArticleDOI
A study of tissue culture cells by electron microscopy : methods and preliminary observations.
TL;DR: By means of a tissue culture technique, cells from chick embryos were procured in a state which proved to be suitable for electron microscopy and disclosed details of cell structure not revealed by other methods of examination.
Journal ArticleDOI
A study of the fine structure of ciliated epithelia
Don W. Fawcett,Keith R. Porter +1 more
Journal ArticleDOI
Cultivation of Trypanosoma cruzi in tissue cultures: a four-year study*
H. Meyer,M. Xavier de Oliveira +1 more
TL;DR: Trypanosoma cruzi is easily cultivated in tissue cultures of chick embryos, but it was not possible to infect chicks with trypanosomes cultivated for more than 2 years in chicken cells.
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