Book ChapterDOI
Enrichment of cardiomyocytes in primary cultures of murine neonatal hearts.
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This protocol describes an efficient and rapid method for isolation and maintenance of long-term cultures of neonatal murine cardiomyocytes by effectively shortening the trypsin enzyme digestion period and theCardiomyocyte enrichment step.Abstract:
In vitro culture of neonatal murine cardiomyocytes is vital for understanding the functions of the heart. Cardiomyocyte cultures are difficult to maintain because they do not proliferate after birth. The maintenance of primary cultures of viable and functional cardiomyocytes is considerably affected by the yield from initial steps of isolation procedures. This protocol describes an efficient and rapid method for isolation and maintenance of long-term cultures of neonatal murine cardiomyocytes by effectively shortening the trypsin enzyme digestion period and the cardiomyocyte enrichment step.read more
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Journal ArticleDOI
Reduction of miR-29a-3p induced cardiac ischemia reperfusion injury in mice via targeting Bax.
Liang Zhang,Jian Zhang,Qiguang Tong,Guannan Wang,Hongling Dong,Zhonglu Wang,Qi Sun,Hangyu Wu +7 more
TL;DR: Reduced miR-29a-3p levels in primary cardiomyocytes contribute to CIR injury-related apoptosis mainly by targeting Bax, according to the authors of the current study.
Journal ArticleDOI
Cardiac-mimetic cell-culture system for direct cardiac reprogramming
Seuk Young Song,Jin Yoo,Seokhyeong Go,Jihye Hong,Hee Su Sohn,Ju-Ro Lee,Mikyung Kang,Gun-Jae Jeong,Seungmi Ryu,Seung Hyun Kim,Nathaniel S. Hwang,Kookheon Char,Byung-Soo Kim +12 more
TL;DR: A cardiac-mimetic cell-culture system that resembles the microenvironment in the heart and provides interactions with cardiomyocytes and electrical cues to the cultured fibroblasts for direct cardiac reprogramming is introduced.
Journal ArticleDOI
An improved two-step method for extraction and purification of primary cardiomyocytes from neonatal mice.
TL;DR: An improved two-step method for extraction and purification of primary cardiomyocytes from neonatal mice exhibited significantly better efficiency in extraction and yielded cells with greater purity and viability.
Journal ArticleDOI
RNA-binding protein CELF1 promotes cardiac hypertrophy via interaction with PEBP1 in cardiomyocytes.
TL;DR: In this article, the expression of CUG triplet repeat-binding protein 1 (CELF1) was significantly increased in pressure overload-induced hypertrophic hearts and angiotensin II (Ang II)-induced neonatal cardiomyocytes.
Posted ContentDOI
A Single Membrane Protein Required for Atrial Secretory Granule Formation
TL;DR: The discovery that atrial myocytes lacking PAM are unable to produce granules and that PAM lacking its monooxygenase activity can rescue granule formation provides new information about the proANP secretory pathway.
References
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Journal ArticleDOI
Induction of manganese superoxide dismutase in rat cardiac myocytes increases tolerance to hypoxia 24 hours after preconditioning.
Nobushige Yamashita,Masashi Nishida,Shiro Hoshida,Tsunehiko Kuzuya,Masatsugu Hori,Naoyuki Taniguchi,T Kamada,Michihiko Tada +7 more
TL;DR: The results suggest that the induction of Mn-SOD in myocytes by preconditioning plays a pivotal role in the acquisition of tolerance to ischemia at a later phase (24 h) of ischemic preconditionsing.
Journal ArticleDOI
Electrophysiological properties of neonatal mouse cardiac myocytes in primary culture.
H B Nuss,Eduardo Marbán +1 more
TL;DR: Neonatal mouse cardiac myocytes in primary culture possess surprisingly large inward currents given the brevity of their action potentials, which support the presence of a low threshold, T‐type Ca2+ current.
Journal ArticleDOI
Neonatal rat cardiomyocytes--a model for the study of morphological, biochemical and electrophysiological characteristics of the heart.
TL;DR: This review examines the methodology for the isolation and cultivation of primary neonatal rat cardiomyocyte cultures, considers possible problems and pitfalls, and presents an optimized protocol for carduomyocyte preparation.
Journal ArticleDOI
Switch from Caspase-dependent to Caspase-independent Death during Heart Development ESSENTIAL ROLE OF ENDONUCLEASE G IN ISCHEMIA-INDUCED DNA PROCESSING OF DIFFERENTIATED CARDIOMYOCYTES
TL;DR: The switch from caspase-dependent to casp enzyme-independent death pathways after cardiac cell differentiation is shown, and the relevance of EndoG in the caspasing-independent DNA processing of differentiated cardiomyocytes is disclosed.
Journal ArticleDOI
Cytotoxicity of cadmium and characteristics of its transport in cardiomyocytes.
Dnyanesh Limaye,Zahir A. Shaikh +1 more
TL;DR: Cadmium is highly toxic to the cardiomyocytes through transport processes that exist for Ca, Zn, and Cu and these involve membrane sulfhydryl groups and include Ca channels.